DiFrancesco Teresa, Boychuk Duane J, Lafferty John D, Crowther Mark A
Hamilton Regional Laboratory Medicine Program, St. Joseph's Hospital, Hamilton, Ontario.
Clin Invest Med. 2012 Jun 1;35(3):E114-6. doi: 10.25011/cim.v35i3.16586.
Preparing bone marrow smears using non-anticoagulated bone marrow aspirate is a traditional practice but many laboratories now use anticoagulated aspirate samples in K-EDTA. There are no published studies comparing the effectiveness of these two methods. This report compares the readability of slides, prepared using non-anticoagulated and anticoagulated methods, from three laboratories in Hamilton Ontario.
A blinded set of 129 aspirate slides prepared using anticoagulated and non-anticoagulated methodologies (using K-EDTA) was reviewed by three reviewers. Slides were classified as unreadable if two of the three observers rejected them based on a standardized survey.
The proportion of slides classed as unreadable varied widely (5.0% to 46.9%) depending on collection and slide preparation methods. Degree of coagulation did not affect readability.
A measurable advantage to using non-anticoagulated bone marrow was not demonstrated. Immediate anticoagulation of bone marrow samples, with laboratory personnel at the bedside to assess sample quality, followed by slide preparation in the laboratory provided the best results.
使用未抗凝的骨髓抽吸物制备骨髓涂片是一种传统做法，但现在许多实验室使用K-EDTA抗凝的抽吸物样本。目前尚无比较这两种方法有效性的已发表研究。本报告比较了安大略省汉密尔顿市三个实验室采用未抗凝和抗凝方法制备的玻片的可读性。
三位审阅者对一组129张采用抗凝和未抗凝方法（使用K-EDTA）制备的抽吸物玻片进行了盲法评估。如果三位观察者中有两位根据标准化调查拒绝某张玻片，则将其归类为不可读。
根据采集和玻片制备方法的不同，归类为不可读的玻片比例差异很大（5.0%至46.9%）。凝血程度不影响可读性。
未证明使用未抗凝骨髓有可衡量的优势。骨髓样本立即抗凝，由实验室人员在床边评估样本质量，然后在实验室制备玻片，可获得最佳结果。
