Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
FEBS Lett. 2012 Jun 4;586(11):1669-73. doi: 10.1016/j.febslet.2012.04.047. Epub 2012 May 3.
Recent studies demonstrated the ability of artificial ribonucleases (aRNases, small organic RNA cleaving compounds) to inactivate RNA-viruses via the synergetic effect of viral RNA cleavage and disruption of viral envelope [1,2]. Herein, we describe the antiviral activity of aRNases against DNA-containing vaccinia virus: screening of aRNases of various structures revealed that amphiphilic compounds built of positively charged 1,4-diazabicyclo[2.2.2] octane substituted at the bridge nitrogen atoms with aliphatic residues efficiently inactivate this virus. The first stage was the destruction of viral membrane and structure of surface proteins (electron microscopy data). Thus, 1,4-diazabicyclo[2.2.2] octane-based aRNases are novel universal agents inactivating both RNA- and DNA-containing viruses.
最近的研究表明，人工核糖核酸酶（aRNases，可切割小分子 RNA 的有机化合物）能够通过协同作用，既能切割病毒 RNA，又能破坏病毒包膜，从而使 RNA 病毒失活[1,2]。在此，我们描述了 aRNases 对含 DNA 的痘病毒的抗病毒活性：对各种结构的 aRNases 的筛选表明，由带正电荷的 1,4-二氮杂双环[2.2.2]辛烷构建的两亲性化合物，其桥氮原子上被脂肪族残基取代，可有效灭活这种病毒。第一阶段是破坏病毒膜和表面蛋白的结构（电子显微镜数据）。因此，基于 1,4-二氮杂双环[2.2.2]辛烷的 aRNases 是新型通用试剂，能够灭活含 RNA 和 DNA 的病毒。
