Fry Simon, Afrough Babak, Leathem Anthony, Dwek Miriam
Department of Molecular and Applied Biosciences, School of Life Sciences, University of Westminster, London, UK.
Methods Mol Biol. 2012;878:267-72. doi: 10.1007/978-1-61779-854-2_18.
Since 2005, lectin microarray technology has emerged as a relatively simple yet powerful technique for the comprehensive analysis of glycoprotein glycosylation. Lectin microarrays represent a new analytical method that can be used to explore the human glycome, a unique source of markers of diseases including cancer. The lectin microarray technology is a sensitive tool with the potential to allow high-throughput analysis of cancer-associated changes in glycosylation. This chapter describes the generation of a lectin-binding signature associated with metastatic primary breast tumours that have been resected, fixed, and embedded in paraffin. Procedures concerning sample and lectin microarray preparation are explained, alongside experimental considerations and approaches to data analysis.
自2005年以来，凝集素微阵列技术已成为一种相对简单却强大的技术，用于对糖蛋白糖基化进行全面分析。凝集素微阵列代表了一种新的分析方法，可用于探索人类糖组，这是包括癌症在内的疾病标志物的独特来源。凝集素微阵列技术是一种灵敏的工具，具有对癌症相关糖基化变化进行高通量分析的潜力。本章描述了与已切除、固定并包埋在石蜡中的转移性原发性乳腺肿瘤相关的凝集素结合特征的生成。解释了有关样品和凝集素微阵列制备的程序，以及实验注意事项和数据分析方法。
