Department of Pneumology & Allergy, Medical University of Lodz, Poland.
Dis Markers. 2012;32(5):329-35. doi: 10.3233/DMA-2011-0890.
Although eicosanoids are involved in lung carcinogenesis they were poorly investigated in exhaled breath condensate (EBC) and bronchoalveolar lavage fluid (BALf) in patients with primary lung cancer. In this study 17 patients with diagnosed non-small cell lung cancer, 10 healthy smokers and 12 healthy nonsmokers were included. The levels of cys-LTs, 8-isoprostane, LTB4 and PGE2 were measured before any treatment in the EBC of all patients and in BALf of patients with lung cancer by enzyme linked immunosorbent assay. 8-isoprostane, LTB4, cys-LTs and PGE2 were detectable in the EBC and BALf. There were no significant differences between healthy smokers and nonsmokers in concentrations of all measured mediators. Compared with both healthy controls, patients with diagnosed lung cancer displayed higher concentrations of cys-LTs (p< 0.05) and LTB4 (p < 0.05) in EBC. In patients with lung cancer, the mean concentrations of all measured mediators were significantly higher in BALf compared with EBC and there was a significant, positive correlation between concentration of cys-LTs, LTB(4) and 8-isoprostane in BALf and their concentrations in the EBC (r=0.64, p < 0.05, r=0.59, p< 0.05, r=0.53, p< 0.05 respectively). Since cys-LT, LTB4 and 8-isoprostane concentrations in EBC from patients with lung cancer reflect their concentrations in BALf, they may serve as a possible non-invasive method to monitor the disease and to assess the effectiveness of therapy.
尽管类二十烷酸参与了肺癌的发生发展，但它们在原发性肺癌患者的呼出气冷凝物（EBC）和支气管肺泡灌洗液（BALf）中的研究甚少。在这项研究中，纳入了 17 名确诊的非小细胞肺癌患者、10 名健康吸烟者和 12 名健康不吸烟者。通过酶联免疫吸附试验，在所有患者的 EBC 中和肺癌患者的 BALf 中，检测了 cys-LTs、8-异前列腺素、LTB4 和 PGE2 的水平，所有患者在任何治疗前均进行了检测。8-异前列腺素、LTB4、cys-LTs 和 PGE2 在 EBC 和 BALf 中均有检测到。健康吸烟者和不吸烟者之间，所有测量介质的浓度均无显著差异。与健康对照组相比，确诊肺癌患者的 EBC 中 cys-LTs（p<0.05）和 LTB4（p<0.05）浓度更高。在肺癌患者中，与 EBC 相比，BALf 中所有测量介质的平均浓度均显著更高，并且 BALf 中 cys-LTs、LTB（4）和 8-异前列腺素的浓度与 EBC 中的浓度呈显著正相关（r=0.64，p<0.05，r=0.59，p<0.05，r=0.53，p<0.05）。由于肺癌患者 EBC 中的 cys-LTs、LTB4 和 8-异前列腺素浓度反映了它们在 BALf 中的浓度，因此它们可能成为一种非侵入性的方法，用于监测疾病并评估治疗效果。
