Department of Neurology, First People's Hospital of Jingzhou, The First Affiliated Hospital of Yangtze University, Jingzhou 434000, People's Republic of China.
Anat Rec (Hoboken). 2012 Jul;295(7):1212-20. doi: 10.1002/ar.22506. Epub 2012 Jun 1.
This study aimed to compare three most widely used surgeries for experimental lumbar spinal drug delivery in rats. The comparison consisted of assessing the effects of the surgeries and evaluating the deficits produced by these three methods. Sprague Dawley rats underwent acute needle puncture, chronic catheterization via laminectomy or nonlaminectomized catheterization. Body weight changes were measured, animals' general and neurological conditions were observed after surgeries, and motor function was examined by Rota Rod test both prior to and post surgery. Furthermore, nociceptive tests were performed to assess the animals' nociception; hematoxylin, and eosin staining of lumbar spinal cord tissue was performed to evaluate local inflammation caused by surgeries; and both lidocaine paralysis detection; and toluidine blue dye assay were used to confirm the exact location of the catheter. Both needle puncture and catheterization via laminectomy had relatively low success rate of surgery and induced various neurological signs; more severe motor dysfunction, hyperalgesia, allodynia, and local inflammation. Nonlaminectomized catheterization had a higher success rate of surgery, and induced only mild agitation, slight cerebral spinal fluid leakage, mild sensory and motor abnormalities, and minimum pathology in the lumbar spinal cord. The nonlaminectomized catheterization used in this study induces a phenotype of less detectable effects on the animal's behavior and is well-tolerated compared to the acute needle puncture and laminectomized catheterization that are widely used in the literature. Nonlaminectomized catheterization is a safe, accurate and effective way for lumbar drug delivery in rats.
本研究旨在比较三种最常用于大鼠实验性腰椎脊髓药物递送的手术。该比较包括评估手术效果和评估这三种方法产生的缺陷。Sprague Dawley 大鼠接受急性针刺、通过椎板切除术或非椎板切除术进行慢性导管插入术。测量体重变化，观察手术后动物的一般和神经状况，并在手术前后通过转棒试验检查运动功能。此外，进行痛觉测试以评估动物的痛觉；对腰椎脊髓组织进行苏木精和伊红染色，以评估手术引起的局部炎症；并进行利多卡因麻痹检测；和甲苯胺蓝染色测定，以确认导管的确切位置。针刺和椎板切除术的导管插入术的手术成功率相对较低，并引起各种神经症状；更严重的运动功能障碍、痛觉过敏、感觉异常和局部炎症。非椎板切除术的导管插入术的手术成功率较高，仅引起轻度激动、轻微脑脊液漏、轻微感觉和运动异常以及腰椎脊髓的最小病理学。与广泛用于文献中的急性针刺和椎板切除术相比，本研究中使用的非椎板切除术引起的动物行为影响较难检测，且耐受性良好。非椎板切除术是一种安全、准确和有效的大鼠腰椎药物递送方法。
