Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, PR China.
J Colloid Interface Sci. 2012 Sep 1;381(1):137-42. doi: 10.1016/j.jcis.2012.05.010. Epub 2012 May 16.
We report here a novel protein sequestration method using polymeric colloidal particles prepared by emulsion polymerization with reactive block copolymers. Specifically, poly(4-vinyl-N-ethylpyridine bromide)-block-polymethylacrylate block copolymers were synthesized from reversible addition-fragmentation chain transfer polymerization and used as emulsifiers for emulsion polymerization of poly(4-vinyl-N-ethylpyridine bromide)-functionalized polymeric colloidal particles. The particles have high and stable zeta potentials when dispersed in solution, regardless of pH variations. As a result, the polymeric colloids demonstrate a high affinity for oppositely charged proteins, even though the isoelectric points of proteins may vary greatly. We show here that BSA can be sequestered highly efficiently with a maximum binding capacity (~900 mg/g). The adsorbed protein is easily released, and the polymeric colloids are regenerated after washing with a buffer solution of high ionic strength. These properties may prompt this type of novel macromolecule-functionalized colloids to be utilized for effective protein adsorption and separation.
我们在这里报告了一种使用通过乳液聚合制备的聚合胶体颗粒的新型蛋白质封闭方法，该聚合胶体颗粒由带有反应性嵌段共聚物的乳液聚合而成。具体而言，通过可逆加成-断裂链转移聚合合成了聚（4-乙烯基-N-乙基吡啶溴化物）-嵌段-聚甲基丙烯酸酯嵌段共聚物，并将其用作聚（4-乙烯基-N-乙基吡啶溴化物）功能化聚合胶体颗粒的乳化剂。无论 pH 值如何变化，这些颗粒在溶液中分散时都具有高且稳定的 ζ 电位。结果，即使蛋白质的等电点可能有很大差异，聚合胶体也对带相反电荷的蛋白质表现出很高的亲和力。我们在这里表明，BSA 可以被高效地封闭，最大结合能力约为 900mg/g。吸附的蛋白质易于释放，并且在用高离子强度的缓冲溶液洗涤后，聚合胶体可以再生。这些特性可能会促使这种新型大分子功能化胶体用于有效蛋白质吸附和分离。
