Department of Plastic and Reconstructive Surgery, Kinki University School of Medicine, Osaka, Japan.
Cells Tissues Organs. 2012;196(5):398-410. doi: 10.1159/000336029. Epub 2012 Jun 5.
We developed a technique to form a bioabsorbable synthetic polymer (polyglycolic acid, PGA) combined with a natural polymer (fibrin) to serve as a scaffold to help retain seeded cells and improve the seeding efficiency of chondrocytes in an implantable construct. This approach was evaluated in a canine autologous implant model of bioengineered cartilage. The implantation site (subcutaneous or intrafascial) and the use of basic fibroblast growth factor (b-FGF) were also evaluated with this system. The intrafascial implantation site yielded optimal results, especially when used in conjunction with fibrin and a b-FGF sustained-release system incorporated into the complex. A thicker, more sustained cartilagenous layer was formed, with a more vascularized outer fibrous supporting tissue layer. This combined approach of implant environment selection, natural polymer for cell retention, and growth factor supplementation offers a more optimized method for generating bioengineered auricular cartilage.
我们开发了一种技术，将可生物吸收的合成聚合物（聚乙醇酸，PGA）与天然聚合物（纤维蛋白）结合在一起，形成一种支架，以帮助保留接种细胞并提高可植入构建体中软骨细胞的接种效率。该方法在犬自体生物工程软骨植入模型中进行了评估。还评估了该系统在皮下或筋膜内植入部位以及碱性成纤维细胞生长因子（b-FGF）的应用。筋膜内植入部位的效果最佳，尤其是与纤维蛋白和结合到复合物中的 b-FGF 缓释系统一起使用时。形成了更厚、更持久的软骨层，并有更具血管化的外部纤维支持组织层。这种选择植入环境、保留细胞的天然聚合物和补充生长因子的综合方法为生成生物工程耳廓软骨提供了一种更优化的方法。
