First Department of Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Oncology. 2012;82(6):341-6. doi: 10.1159/000338327. Epub 2012 Jun 2.
Cytological examination of samples obtained by bronchoscopy is a useful method for establishing the diagnosis of non-small cell lung cancer (NSCLC). However, the utility of a highly sensitive method for the detection of epidermal growth factor receptor (EGFR) mutation in the cytological specimens has not been fully evaluated.
We retrospectively examined the efficacy of the peptide nucleic acid-locked nucleic acid polymerase chain reaction (PNA-LNA PCR) clamp method for detecting EGFR mutations in 122 bronchoscopic cytological specimens from NSCLC patients.
Overall, 41 specimens (33.6%) were positive for EGFR mutation. Twenty-nine (39.7%) of 73 specimens obtained by using endobronchial ultrasonography with a guide sheath, 7 (33.3%) of 21 specimens obtained under direct vision by using a conventional bronchoscope, 4 (36.4%) of 11 specimens obtained by using an ultrathin bronchoscope, and 1 (5.9%) of 17 specimens obtained by endobronchial ultrasound-guided transbronchial needle aspiration were positive for EGFR mutation. Furthermore, among 22 resected NSCLC cases, the EGFR mutation status obtained from bronchoscopic materials was consistent with the status obtained from surgical samples, with the exception of 1 case.
The detection of EGFR mutation by subjecting bronchoscopic cytological specimens to a PNA-LNA PCR clamp assay proves useful.
通过支气管镜获取的样本细胞学检查是诊断非小细胞肺癌（NSCLC）的有用方法。然而，尚未充分评估针对表皮生长因子受体（EGFR）突变的高敏感检测方法在细胞学标本中的应用。
我们回顾性分析了肽核酸锁核酸聚合酶链反应（PNA-LNA PCR）夹心法检测 122 例 NSCLC 患者支气管镜细胞学标本中 EGFR 突变的疗效。
总体而言，41 份标本（33.6%）EGFR 突变阳性。29 份（39.7%）经支气管内超声引导鞘内支气管镜获取的标本、7 份（33.3%）经常规支气管镜直视下获取的标本、4 份（36.4%）经超细支气管镜获取的标本和 17 份经支气管内超声引导经支气管针吸活检获取的标本 EGFR 突变阳性。此外，22 例 NSCLC 切除病例中，支气管镜取材的 EGFR 突变状态与手术样本一致，除 1 例外。
对支气管镜细胞学标本进行 PNA-LNA PCR 夹心法检测 EGFR 突变具有一定的临床应用价值。
