National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-8604, Japan.
Appl Microbiol Biotechnol. 2013 Apr;97(7):2951-9. doi: 10.1007/s00253-012-4188-8. Epub 2012 Jun 8.
Pseudozyma antarctica JCM 10317 exhibits a strong degradation activity for biodegradable plastics (BPs) such as agricultural mulch films composed of poly(butylene succinate) (PBS) and poly(butylene succinate-co-adipate) (PBSA). An enzyme named PaE was isolated and the gene encoding PaE was cloned from the strain by functional complementation in Saccharomyces cerevisiae. The deduced amino acid sequence of PaE contains 198 amino acids with a predicted molecular weight of 20,362.41. High identity was observed between this sequence and that of cutinase-like enzymes (CLEs) (61-68%); therefore, the gene encoding PaE was named PaCLE1. The specific activity of PaE against emulsified PBSA was 54.8±6.3 U/mg. In addition to emulsified BPs, PaE degraded solid films of PBS, PBSA, poly(ε-caprolactone), and poly(lactic acid).
南极假丝酵母 JCM 10317 对可生物降解塑料（BPs）具有很强的降解活性，如由聚丁二酸丁二醇酯（PBS）和聚丁二酸丁二醇酯-己二酸酯（PBSA）组成的农用覆盖膜。从该菌株中通过在酿酒酵母中的功能互补分离出一种名为 PaE 的酶，并克隆了编码 PaE 的基因。PaE 的推导氨基酸序列包含 198 个氨基酸，预测分子量为 20362.41。该序列与角质酶样酶（CLEs）（61-68%）之间观察到高度的同一性；因此，编码 PaE 的基因被命名为 PaCLE1。PaE 对乳化 PBSA 的比活性为 54.8±6.3 U/mg。除了乳化的 BPs 外，PaE 还降解 PBS、PBSA、聚己内酯和聚乳酸的固体薄膜。
