Wu Shuyan, Hooks David, Brightwell Gale
AgResearch Ltd., Grasslands, Palmerston North 4442, New Zealand.
New Zealand Food Safety Science and Research Centre, Tennent Drive, Massey University, Palmerston North 4474, New Zealand.
Bioengineering (Basel). 2025 Jan 14;12(1):68. doi: 10.3390/bioengineering12010068.
Enzymatic depolymerisation is increasingly recognised as a reliable and environmentally friendly method. The development of this technology hinges on the availability of high-quality enzymes and associated bioreaction systems for upscaling biodegradation. Microbial heterologous expression systems have been studied for meeting this demand. Among these systems, the expression system has emerged as a widely used platform for producing secreted heterologous proteins. This article provides an overview of studies involving the recombinant expression of polymer-degrading enzymes using the expression system. Research on expression of interested enzymes with depolymerising ability, including cutinase, lipase, and laccase, are highlighted in the review. The key factors influencing the heterologous expression of polymer-degrading enzymes in are discussed, shedding light on the challenges and opportunities in the development of depolymerising biocatalysts through the expression system.
酶促解聚日益被认为是一种可靠且环保的方法。该技术的发展取决于高质量酶的可用性以及用于扩大生物降解规模的相关生物反应系统。人们已经研究了微生物异源表达系统来满足这一需求。在这些系统中，[具体表达系统名称]表达系统已成为生产分泌型异源蛋白的广泛使用平台。本文概述了使用[具体表达系统名称]表达系统进行聚合物降解酶重组表达的研究。综述中重点介绍了对具有解聚能力的感兴趣酶的[具体表达系统名称]表达研究，包括角质酶、脂肪酶和漆酶。讨论了影响[具体表达系统名称]中聚合物降解酶异源表达的关键因素，揭示了通过[具体表达系统名称]表达系统开发解聚生物催化剂所面临的挑战和机遇。
