van Vianen P H, Thaithong S, Reinders P P, van Engen A, van der Keur M, Tanke H J, van der Kaay H J, Mons B
University of Leiden, The Netherlands.
Am J Trop Med Hyg. 1990 Dec;43(6):602-7. doi: 10.4269/ajtmh.1990.43.602.
A method is described for the fully automated reading of Plasmodium falciparum drug susceptibility tests. Cultured material was fixed and could be stored for greater than or equal to 6 months until analysis. The parasites were stained for DNA with the fluorescent dye Hoechst 33258 and analyzed by flow cytometry. The procedure was done in 96-well microtiter plates, after which the material was directed through the sensing region in the flow cytometer. The data resulting from the analysis were stored by microcomputer and processed by a program developed for this purpose. Using this method, a number of different parameters describing the growth in culture can be assessed.
描述了一种用于全自动读取恶性疟原虫药物敏感性试验的方法。培养物固定后可保存大于或等于6个月直至分析。用荧光染料Hoechst 33258对寄生虫的DNA进行染色，并用流式细胞仪进行分析。该程序在96孔微量滴定板中进行，之后将材料引导通过流式细胞仪的传感区域。分析得到的数据由微型计算机存储，并通过为此目的开发的程序进行处理。使用这种方法，可以评估描述培养物生长的许多不同参数。
