Li Sha-Sha, Lu Chuan-Jian, Jiang Zhi-Ting
Molecular Biology Laboratory, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2012 May;32(5):633-8.
To investigate the contents changes of potential biomarkers of patients infected with influenza A (H1N1) virus of different Chinese medicine (CM) syndrome types.
Eighty-two patients with influenza A (H1N1) virus were differentiated as three syndrome types, i. e., wind-heat invading weifen syndrome (51 cases), heat-toxicity attacking Fei syndrome (22 cases), and superficies tightened by wind cold syndrome (9 cases) according to Chinese medicine syndrome typing. According to patients' willingness and clinical conditions, they were treated by three therapeutic schedules, i. e., herbal therapy, symptomatic treatment, and antiviral therapy. The changes of potential biomarkers contents were detected in the serum of patients of various syndrome types before and after treatment. Results There was no statistical difference in the potential biomarkers contents correlated to symptoms of fever, inflammation and cough, such as PGG2, 20-COOH-LTB4, homocystein, and so on in the serum of patients of various syndrome types before treatment (P > 0.05). There was statistical difference in the potential biomarkers such as 20-OH-LTE4, LTA4, and linolenic acid, etc. between superficies tightened by wind cold syndrome and wind-heat invading weifen syndrome (P < 0.05, P < 0.01). There was statistical difference in the potential biomarkers such as PGF1alpha, prostanoic acid, and etc. between superficies tightened by wind cold syndrome and heat-toxicity attacking Fei syndrome (P < 0.05, P < 0.01). Statistical difference existed in other indices other than dUTP; 5,10-methylene-THF and PGF1alpha in wind-heat invading weifen syndrome and superficies tightened by wind cold syndrome; prostanoic acid, homocysteine, and glucose in superficies tightened by wind cold syndrome when compared with before treatment in the same group (P < 0.05, P < 0.01). The changing tendency of potential biomarkers among different syndrome types was identical. Of them, the change of 6-keto-PGF1alpha content was the most obviously of all indices.
There was difference in the contents of potential biomarkers of patients infected with influenza A (H1N1) virus of different syndrome types, and our study provided experimental data support for the objectiveness of CM syndrome differentiation from the perspective of metabolic substances.
探讨甲型H1N1流感病毒感染患者不同中医证型潜在生物标志物含量变化。
将82例甲型H1N1流感病毒感染患者按中医辨证分为风热犯卫证（51例）、热毒袭肺证（22例）、风寒束表证（9例）3种证型。根据患者意愿及临床情况，采用中药治疗、对症治疗、抗病毒治疗3种治疗方案。检测各证型患者治疗前后血清中潜在生物标志物含量变化。结果各证型患者治疗前血清中与发热、炎症、咳嗽等症状相关的潜在生物标志物如PGG2、20-COOH-LTB4、同型半胱氨酸等含量比较，差异无统计学意义（P>0.05）。风寒束表证与风热犯卫证之间，20-OH-LTE4、LTA4、亚麻酸等潜在生物标志物差异有统计学意义（P<0.05，P<0.01）。风寒束表证与热毒袭肺证之间，PGF1α、前列腺酸等潜在生物标志物差异有统计学意义（P<0.05，P<0.01）。同一组中，风寒束表证治疗后除dUTP外，5,10-亚甲基-THF、PGF1α等其他指标，风热犯卫证与风寒束表证比较，前列腺酸、同型半胱氨酸、葡萄糖等，与治疗前比较差异有统计学意义（P<0.05，P<0.01）。不同证型潜在生物标志物变化趋势一致。其中，6-酮-PGF1α含量变化最为明显。
甲型H1N1流感病毒感染患者不同证型潜在生物标志物含量存在差异，本研究从代谢物质角度为中医辨证的客观性提供了实验数据支持。
