Kochevenko A S, Fernie A R
Ukr Biokhim Zh (1999). 2012 Jan-Feb;84(1):18-25.
This study presents evidence for the role of BCAT3 and BCAT4 proteins in the synthesis of branched-chain-amino-acids in tomato Solanum lycopersicum. BCAT3 and BCAT4 genes were located on tomato chromosomal map by RFLP method (restriction fragment length polymorphism). Using confocal microscopy it was shown that BCAT3-GFP and BCAT4-GFP fusion proteins were localised in chloroplasts. It was observed that these aminotransferase isoforms exhibited distinct kinetic properties and a differential expression pattern of mRNA levels in various tomato tissues.
本研究提供了BCAT3和BCAT4蛋白在番茄（Solanum lycopersicum）支链氨基酸合成中作用的证据。通过限制性片段长度多态性（RFLP）方法将BCAT3和BCAT4基因定位到番茄染色体图谱上。利用共聚焦显微镜观察发现，BCAT3-GFP和BCAT4-GFP融合蛋白定位于叶绿体中。研究观察到，这些转氨酶同工型在不同番茄组织中表现出不同的动力学特性和mRNA水平的差异表达模式。
