Pfaffenbach G M, Melvold R W, Nathenson S G
Department of Microbiology, Albert Einstein College of Medicine, Bronx, New York 10461.
Biochem Genet. 1990 Aug;28(7-8):433-41. doi: 10.1007/BF02401430.
The "bg" series of MHC mutations is the most prevalent type of mutations of Kb in C57BL/6 mice screened by reciprocal tail skin grafting. The basis for identification of this series of mutations is the incompatibility of grafts between the parental B6 and the mutant. This series takes the longest to reciprocally reject the skin grafts. The series can be subdivided into "bg 1" and "bg 2" groups based on Kb-restricted recognition of virus-infected mutant target cells. The biochemical basis for these mutations are amino acid substitutions at residues 116 and 121 of the Kb transplantation antigen. These substitutions do not alter monoclonal antibody binding sites. The structural basis of MAb binding and the genetic basis of the mutation are discussed.
MHC突变的“bg”系列是通过相互尾皮移植筛选出的C57BL/6小鼠中Kb最普遍的突变类型。鉴定该系列突变的依据是亲代B6与突变体之间移植物的不相容性。该系列相互排斥皮肤移植物所需的时间最长。根据Kb对病毒感染的突变靶细胞的限制性识别，该系列可细分为“bg 1”和“bg 2”组。这些突变的生化基础是Kb移植抗原第116和121位残基的氨基酸取代。这些取代不会改变单克隆抗体结合位点。讨论了单克隆抗体结合的结构基础和突变的遗传基础。
