Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University, Baoding 071002, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Jul 1;900:18-23. doi: 10.1016/j.jchromb.2012.05.021. Epub 2012 May 24.
A highly selective molecularly imprinted solid phase extraction (MISPE) coupled with liquid chromatography-ultraviolet detection was developed for the determination of salbutamol (SAL) in ham sausages. New molecularly imprinted polymers (MIPs) were synthesized with phenylephrine as dummy template and it revealed good affinity to SAL in methanol-acetonitrile system. Adsorption capacity of the MIPs was evaluated by dynamic adsorption experiments. The MIPs were used as SPE sorbent for the selective clean-up and pre-concentration of SAL in ham sausage samples. The results showed that the matrix compounds presented in ham sausage samples could be removed effectively and the recoveries of SAL at three spiked levels were ranged from 82.6 to 100.5% with the relative standard deviation (RSD) of less than 3.6%. This method is simple and sensitive, and is therefore an alternative tool to the existing methods for analyzing residual SAL in biological samples.
建立了一种高效选择性分子印迹固相萃取（MISPE）- 高效液相色谱-紫外检测法，用于测定火腿肠中的沙丁胺醇（SAL）。以苯肾上腺素为模板分子合成了新型分子印迹聚合物（MIPs），在甲醇-乙腈体系中对 SAL 具有良好的亲和性。通过动态吸附实验评价了 MIPs 的吸附性能。将 MIPs 用作 SPE 吸附剂，用于火腿肠样品中 SAL 的选择性净化和预浓缩。结果表明，该方法能有效去除基质化合物，在 3 个加标水平下，SAL 的回收率在 82.6%至 100.5%之间，相对标准偏差（RSD）小于 3.6%。该方法简单灵敏，是分析生物样品中残留 SAL 的现有方法的一种替代手段。
