Marsh J B, Sparks C E
Metabolism. 1979 Oct;28(10):1040-5. doi: 10.1016/0026-0495(79)90008-8.
Experimental nephrosis was induced in rats by administration of puromycin aminonucleoside and the levels of plasma lipoproteins were examined 7 days later and compared to controls. As determined by density ultracentrifugation, VLDL, IDL, LDL, and HDL protein levels were increased by 8, 4, 5, and 5 times, respectively. These increases were accompanied by changes in lipid and apoprotein composition. The VLDL, IDL, and LDL fractions contained less triglyceride and more phospholipid and cholesterol, while HDL lipid composition was not altered. The apoprotein composition of VLDL and IDL were not measurably altered, but LDL contained less apoE. HDL had a markedly abnormal composition characterized by an almost complete absence of apoA-IV and apoE, increased apoA-1, and decreased apoC. While increased hepatic synthesis can account for much of the observed hyperlipoproteinemia in nephrosis, the changes in lipoprotein composition suggest impaired catabolism as a contributory factor.
通过给予嘌呤霉素氨基核苷在大鼠中诱导实验性肾病，并在7天后检测血浆脂蛋白水平并与对照组进行比较。通过密度超速离心法测定，极低密度脂蛋白（VLDL）、中间密度脂蛋白（IDL）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）的蛋白质水平分别增加了8倍、4倍、5倍和5倍。这些增加伴随着脂质和载脂蛋白组成的变化。VLDL、IDL和LDL组分中甘油三酯含量减少，磷脂和胆固醇含量增加，而HDL的脂质组成未改变。VLDL和IDL的载脂蛋白组成没有明显改变，但LDL中载脂蛋白E含量减少。HDL的组成明显异常，其特征是几乎完全缺乏载脂蛋白A-IV和载脂蛋白E，载脂蛋白A-1增加，载脂蛋白C减少。虽然肝脏合成增加可解释肾病中观察到的大部分高脂蛋白血症，但脂蛋白组成的变化表明分解代谢受损是一个促成因素。
