Department of Rehabilitation Medicine, College of Medicine, Hallym University, Republic of Korea.
Burns. 2012 Nov;38(7):1066-71. doi: 10.1016/j.burns.2012.02.016. Epub 2012 Jun 7.
No study has reported an animal-based experimental model of electrical injury to the spinal cord. This paper presents the first systematic establishment of an animal model of electrical injury to the spinal cord with subsequent pathophysiologic analysis.
The voltage required for the electrical shock was generated by an electroconvulsive therapy apparatus (57800 ECT unit; UGO BASILE, Italy). We used one side ear as the entry site and the contralateral hind limb as the exit site. Seven electrical shock (frequency, 120 Hz; pulse width, 0.9 ms; duration, 3 s; current, 99 mA) was applied to each rat and used rat showing hind limb weakness. Radiologic and histologic evaluations were performed at one day, one, two and four weeks after injury.
Twelve rats showed the hind limb weakness among the total 18 rats. Manganese-enhanced magnetic resonance imaging showed interruption of spinal cord enhancement in the thoracic area. Histological examination showed a greater decrease in the number of neurons in the ventral horn versus the dorsal horn.
This study demonstrates a novel design and analysis of an animal-based experimental model of spinal cord injury by electrical etiology. This model is useful for experimental studies of injuries to the spinal cord.
目前尚无关于脊髓电损伤动物模型的研究。本文首次系统建立了一种基于动物的脊髓电损伤模型，并进行了后续的病理生理分析。
电休克仪（57800ECT 仪；意大利 UGO BASILE）产生所需的电压。我们使用一侧耳朵作为入口部位，另一侧后肢作为出口部位。对每只大鼠施加 7 次电刺激（频率 120Hz，脉冲宽度 0.9ms，持续时间 3s，电流 99mA），直到大鼠出现后肢无力。在损伤后 1 天、1 周、2 周和 4 周进行放射学和组织学评估。
在 18 只大鼠中，有 12 只出现了后肢无力。锰增强磁共振成像显示胸段脊髓增强中断。组织学检查显示，腹角神经元数量较背角减少更多。
本研究展示了一种新型的基于动物的电损伤性脊髓损伤实验模型的设计和分析。该模型可用于脊髓损伤的实验研究。
