肝脏中两种不同的HNF1样转录激活因子与大鼠醛缩酶B启动子的同一区域结合。

Two different HNF1-like transcription activators in the liver bind to the same region of the rat aldolase B promoter.

作者信息

Ito K, Tanaka T, Tsutsumi R, Ishikawa K, Tsutsumi K

机构信息

Department of Biochemistry, Yamagata University School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Dec 31;173(3):1337-43. doi: 10.1016/s0006-291x(05)80934-9.

DOI:10.1016/s0006-291x(05)80934-9

PMID:2268334

Abstract

Coexistence of two different HNF1-like factors (AlF-A1 and AlF-A2) in rat liver is described. The two factors had similar molecular weight and the same sequence-specificity in DNA-binding. One of these factors AlF-A1 was retained on wheat germ agglutinin-sepharose column and eluted from it with N-acetylglucosamine, while AlF-A2 was not, suggesting that AlF-A1 is glycosylated. Using rat brain nuclear extract, both factors stimulated transcription in vitro, but their stimulatory effects differed from each other.

摘要

本文描述了大鼠肝脏中两种不同的HNF1样因子（AlF-A1和AlF-A2）的共存情况。这两种因子具有相似的分子量，且在DNA结合方面具有相同的序列特异性。其中一种因子AlF-A1可保留在麦胚凝集素-琼脂糖柱上，并用N-乙酰葡糖胺从该柱上洗脱下来，而AlF-A2则不能，这表明AlF-A1是糖基化的。使用大鼠脑细胞核提取物，这两种因子均可在体外刺激转录，但它们的刺激作用彼此不同。