Oil Technology Section, Dept. of Chemical Technology, University College of Science & Technology, University of Calcutta, Kolkata 700 009, India.
Food Chem Toxicol. 2012 Aug;50(8):2811-8. doi: 10.1016/j.fct.2012.05.038. Epub 2012 Jun 7.
The present study was undertaken to evaluate under in vivo condition the effects of α-linolenic acid and α-eleostearic acid against methyl mercury (MeHg) induced oxidative stress. Male albino rats were divided into six groups. Group 1 was under normal control and Group 2 was treated with methyl mercury chloride (MeHgCl; 5 mg/kgBW/day). Groups 3, 4, 5 and 6 were orally treated with different doses of the two fatty acids (0.5% and 1.0% of total lipid given for each kind of linolenic acid isomer) along with MeHgCl (5mg/kgBW). Comet assay of blood lymphocytes showed that administration of α-linolenic acid reduced DNA damage significantly (P<0.05). Results also showed that activity of antioxidant enzymes of plasma and brain tissue and total antioxidant capacity in plasma decreased significantly due to oxidative stress generated by MeHgCl. Administration in higher dose of both kind of linolenic acid restored all the activities of the antioxidant enzymes and also reduced lipid peroxidation and increased total antioxidant capacity in plasma. Both kinds of linolenic acid successfully maintained the RBC membrane integrity which was totally disrupted and became flat due to MeHgCl stress. α-Linolenic acid was more efficient antioxidant than α-eleostearic acid against oxidative DNA damage.
本研究旨在体内条件下评估 α-亚麻酸和 α-桐酸对甲基汞（MeHg）诱导的氧化应激的影响。雄性白化大鼠分为六组。第 1 组为正常对照组，第 2 组给予甲基汞氯化物（MeHgCl；5mg/kgBW/天）。第 3、4、5 和 6 组分别用两种脂肪酸（亚麻酸异构体的 0.5%和 1.0%总脂质）和 MeHgCl（5mg/kgBW）口服治疗。血液淋巴细胞的彗星试验表明，α-亚麻酸的给药可显著降低 DNA 损伤（P<0.05）。结果还表明，由于 MeHgCl 产生的氧化应激，血浆和脑组织抗氧化酶的活性以及血浆中的总抗氧化能力显著降低。两种亚麻酸的高剂量给药均恢复了所有抗氧化酶的活性，并降低了血浆中的脂质过氧化作用，增加了总抗氧化能力。两种亚麻酸都成功地维持了红细胞膜的完整性，由于 MeHgCl 应激，红细胞膜完全破裂并变得平坦。α-亚麻酸对氧化 DNA 损伤的抗氧化作用比 α-桐酸更有效。
