成年小鼠尿液中前表皮生长因子和高分子量表皮生长因子的鉴定。

Identification of pro-epidermal growth factor and high molecular weight epidermal growth factors in adult mouse urine.

作者信息

Lakshmanan J, Salido E C, Lam R, Barajas L, Fisher D A

机构信息

Department of Pediatrics and Pathology, Harbor UCLA Medical Center, UCLA School of Medicine, Torrance 90509.

出版信息

Biochem Biophys Res Commun. 1990 Dec 31;173(3):902-11. doi: 10.1016/s0006-291x(05)80871-x.

DOI:10.1016/s0006-291x(05)80871-x

PMID:2268351

Abstract

By use of immunoblot analysis, we demonstrate the presence of a pro-Epidermal growth factor (EGF) with an approximate molecular weight of 165 kDa in adult mouse urine. In addition, urine contains four high molecular weight EGFs with approximate molecular weights of 116, 97, 66 and 56 kDa. The 165 kDa pro-EGF as well as the 66 and 56 kDa EGFs also are detectable in mouse kidney extract. Neither urine nor kidney contain the mature EGF of 6 kDa. The 165 kDa pro-EGF is the major product synthesized in renal tissue and secreted in urine. The finding of high molecular weight EGFs in urine suggests that part of pro-EGF secreted into urine undergoes partial proteolysis distal to its site of synthesis.

摘要

通过免疫印迹分析，我们证实在成年小鼠尿液中存在一种分子量约为165 kDa的前表皮生长因子（EGF）。此外，尿液中还含有四种高分子量的EGF，分子量约为116、97、66和56 kDa。在小鼠肾脏提取物中也可检测到165 kDa的前EGF以及66和56 kDa的EGF。尿液和肾脏中均未检测到6 kDa的成熟EGF。165 kDa的前EGF是肾组织合成并分泌到尿液中的主要产物。尿液中高分子量EGF的发现表明，分泌到尿液中的部分前EGF在其合成部位远端发生了部分蛋白水解。