School of Biotechnology, National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland.
Biochem Biophys Res Commun. 2012 Jul 6;423(3):473-7. doi: 10.1016/j.bbrc.2012.05.144. Epub 2012 Jun 5.
A novel gold nanoarray (NA)-based platform was developed for microarray applications. This novel approach is based upon the principle of nanosphere lithography and can be used for one-step antibody immobilization. The developed platform was checked by functionalizing with cysteine followed by capturing biotinylated antibody and detecting it with dye-conjugated steptravidin. An immunoassay was performed with spiked samples containing human fetuin A antigen. The minimum limits of detection (LOD) of human fetuin A for NA-based and conventional microarray platforms were 50 pg/mL and 50 ng/mL, respectively. The developed approach was highly reproducible and unlike conventional microarray approaches the use of a spotting system was omitted because immobilization was controlled and directed on the predefined arrays. This approach could be an ideal alternative for developing microarrays. And, the ease of the strategy also allows the high throughput production of the microarrays.
一种新型的基于金纳米阵列（NA）的平台被开发用于微阵列应用。这种新方法基于纳米球光刻的原理，可以用于一步抗体固定化。通过巯基化，然后捕获生物素化抗体，并用染料标记的链霉亲和素进行检测，来检查开发的平台。用人胎球蛋白 A 抗原的加标样品进行免疫测定。基于 NA 的和传统微阵列平台检测人胎球蛋白 A 的最小检测限（LOD）分别为 50pg/ml 和 50ng/ml。所开发的方法具有高度的重现性，与传统的微阵列方法不同，因为省略了点样系统，因为固定化是在预定义的阵列上进行控制和定向的。这种方法可能是开发微阵列的理想选择。而且，该策略的简便性还允许微阵列的高通量生产。
