Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Mod Pathol. 2012 Nov;25(11):1526-33. doi: 10.1038/modpathol.2012.103. Epub 2012 Jun 8.
Squamous cell carcinoma of the urinary bladder is unusual and of unknown etiology. There is a well-established association between human papillomavirus (HPV) infection and the development of cervical and head/neck squamous cell carcinomas. However, the role of HPV in the pathogenesis of squamous cell carcinoma of the urinary bladder is uncertain. The purposes of this study were to investigate the possible role of HPV in the development of squamous cell carcinoma of the urinary bladder and to determine if p16 expression could serve as a surrogate marker for HPV in this malignancy. In all, 42 cases of squamous cell carcinoma of the urinary bladder and 27 cases of urothelial carcinoma with squamous differentiation were investigated. HPV infection was analyzed by both in situ hybridization at the DNA level and immunohistochemistry at the protein level. p16 protein expression was analyzed by immunohistochemistry. HPV DNA and protein were not detected in 42 cases of squamous cell carcinoma (0%, 0/42) or 27 cases of urothelial carcinoma with squamous differentiation (0%, 0/15). p16 expression was detected in 13 cases (31%, 13/42) of squamous cell carcinoma and 9 cases (33%, 9/27) of urothelial carcinoma with squamous differentiation. There was no correlation between p16 expression and the presence of HPV infection in squamous cell carcinoma of the bladder or urothelial carcinoma with squamous differentiation. Our data suggest that HPV does not play a role in the development of squamous cell carcinoma of the urinary bladder or urothelial carcinoma with squamous differentiation. p16 expression should not be used as a surrogate marker for evidence of HVP infection in either squamous cell carcinoma of the urinary bladder or urothelial carcinoma with squamous differentiation as neither HVP DNA nor protein is detectable in these neoplasms.
膀胱鳞状细胞癌较为罕见，其病因不明。人乳头瘤病毒（HPV）感染与宫颈癌和头颈部鳞状细胞癌的发生发展密切相关。然而，HPV 在膀胱鳞状细胞癌发病机制中的作用尚不确定。本研究旨在探讨 HPV 在膀胱鳞状细胞癌发生发展中的可能作用，并确定 p16 表达是否可作为该肿瘤中 HPV 的替代标志物。共检测了 42 例膀胱鳞状细胞癌和 27 例具有鳞状分化的尿路上皮癌病例。采用原位杂交法在 DNA 水平和免疫组化法在蛋白水平检测 HPV 感染，免疫组化法分析 p16 蛋白表达。在 42 例膀胱鳞状细胞癌（0%，0/42）或 27 例具有鳞状分化的尿路上皮癌（0%，0/15）中均未检测到 HPV DNA 和蛋白。13 例（31%，13/42）膀胱鳞状细胞癌和 9 例（33%，9/27）具有鳞状分化的尿路上皮癌中检测到 p16 表达。膀胱鳞状细胞癌或具有鳞状分化的尿路上皮癌中 p16 表达与 HPV 感染的存在均无相关性。我们的数据表明，HPV 并未参与膀胱鳞状细胞癌或具有鳞状分化的尿路上皮癌的发生发展。p16 表达不应作为膀胱鳞状细胞癌或具有鳞状分化的尿路上皮癌中 HPV 感染的替代标志物，因为在这些肿瘤中均无法检测到 HPV DNA 或蛋白。
