一种消除色谱聚焦缓冲液并浓缩线粒体苹果酸脱氢酶次要活性亚形式的快速方法。
A rapid procedure for eliminating chromatofocusing buffer and concentrating minor active subforms of mitochondrial malate dehydrogenase.
作者信息
Gelpí J L, Gracia V, Imperial S, Mazo A, Cortés A
机构信息
Department de Bioqúimica i Fisiologia, Facultat de Química, Universitat de Barcelona, Spain.
出版信息
Biotechniques. 1990 Nov;9(5):616-8.
PMID:2268429
Abstract
Mitochondrial malate dehydrogenase from several sources contains different molecular forms whose origin is still under discussion. Separation of these subforms has been achieved by chromatofocusing. A simple and rapid method, based on 5' AMP Sepharose chromatography, has been developed to concentrate mitochondrial malate dehydrogenase subforms and simultaneously remove chromatofocusing buffer.
摘要
来自多种来源的线粒体苹果酸脱氢酶含有不同的分子形式，其来源仍在讨论之中。这些亚形式已通过色谱聚焦法分离出来。一种基于5'-AMP琼脂糖层析的简单快速方法已被开发出来，用于浓缩线粒体苹果酸脱氢酶亚形式并同时去除色谱聚焦缓冲液。
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