Nakamura H, Ozawa H
Department of Oral Anatomy, Niigata University School of Dentistry, Japan.
Arch Histol Cytol. 1990 Oct;53(4):351-69. doi: 10.1679/aohc.53.351.
Lectin cytochemistry was carried out to elucidate the role of stratum intermedium cells and papillary layer cells in amelogenesis, especially in the process of sugar incorporation and on membrane characteristics according to their cytodifferentiation. Regarding the lectin-reaction on the plasma membrane, little or at best a weak reaction of Con A, UEA-I, PNA, MPA and WGA was seen in stratum intermedium cells from the late differentiation stage to the early secretory stage of ameloblasts. Lectin-stainability in the stratum intermedium increased in accordance with the cytodifferentiation of ameloblasts. At the active secretory stage of ameloblasts, lectins intensely stained the plasma membranes of stratum intermedium cells. The plasma membranes of papillary layer cells at both stages of ruffle-ended and smooth-ended ameloblasts were stained by same lectins as well. The results therefore suggest that: 1) stratum intermedium cells bring about changes in the glycolipids and glycoproteins of their plasma membranes in accordance with the cytodifferentiation of ameloblasts; 2) they regulate the transport of mineral and/or organic materials between ameloblasts and extracellular fluid via highly charged plasma membranes generated by glycocalyx; 3) the cell-cell interaction of stratum intermedium cells with ameloblasts, in which carbohydrate-protein (endogenous lectin) interaction plays a significant role, is important for the cytodifferentiation of these cells. Regarding the papillary layer cells, the results suggest that they also regulate the transport of minerals by their charged plasma membranes and participate in the removal of the enamel matrix.
采用凝集素细胞化学方法来阐明中间层细胞和乳头层细胞在釉质形成中的作用，特别是在糖掺入过程以及根据其细胞分化的膜特性方面的作用。关于质膜上的凝集素反应，在成釉细胞从分化后期到分泌早期的中间层细胞中，几乎看不到或最多只能看到刀豆球蛋白A（Con A）、荆豆凝集素I（UEA-I）、花生凝集素（PNA）、麦芽凝集素（MPA）和小麦胚凝集素（WGA）的微弱反应。随着成釉细胞的细胞分化，中间层的凝集素染色性增加。在成釉细胞的活跃分泌期，凝集素强烈染色中间层细胞的质膜。在成釉细胞的皱襞端和平滑端两个阶段，乳头层细胞的质膜也被相同的凝集素染色。因此，结果表明：1）中间层细胞根据成釉细胞的细胞分化，使其质膜上糖脂和糖蛋白发生变化；2）它们通过糖萼产生的高电荷质膜调节成釉细胞与细胞外液之间矿物质和/或有机物质的运输；3）中间层细胞与成釉细胞之间的细胞间相互作用对这些细胞的细胞分化很重要，其中碳水化合物-蛋白质（内源性凝集素）相互作用起重要作用。关于乳头层细胞，结果表明它们也通过其带电的质膜调节矿物质的运输，并参与釉质基质的去除。
