Laboratory of Genetics, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, the Netherlands.
Biosystematics Group, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, the Netherlands.
New Phytol. 2012 Aug;195(3):688-698. doi: 10.1111/j.1469-8137.2012.04195.x. Epub 2012 Jun 11.
Chromosomal rearrangements are relatively rare evolutionary events and can be used as markers to study karyotype evolution. This research aims to use such rearrangements to study chromosome evolution in Solanum. Chromosomal rearrangements between Solanum crops and several related wild species were investigated using tomato and potato bacterial artificial chromosomes (BACs) in a multicolour fluorescent in situ hybridization (FISH). The BACs selected are evenly distributed over seven chromosomal arms containing inversions described in previous studies. The presence/absence of these inversions among the studied Solanum species were determined and the order of the BAC-FISH signals was used to construct phylogenetic trees.Compared with earlier studies, data from this study provide support for the current grouping of species into different sections within Solanum; however, there are a few notable exceptions, such as the tree positions of S. etuberosum (closer to the tomato group than to the potato group) and S. lycopersicoides (sister to S. pennellii). These apparent contradictions might be explained by interspecific hybridization events and/or incomplete lineage sorting. This cross-species BAC painting technique provides unique information on genome organization, evolution and phylogenetic relationships in a wide variety of species. Such information is very helpful for introgressive breeding.
染色体重排是相对罕见的进化事件，可作为研究染色体进化的标记。本研究旨在利用这些重排来研究茄属植物的染色体进化。使用番茄和马铃薯的细菌人工染色体 (BAC) 进行多色荧光原位杂交 (FISH)，研究了茄属作物与几种相关野生种之间的染色体重排。选择的 BAC 均匀分布在 7 条染色体臂上，这些染色体臂包含先前研究中描述的倒位。确定了这些倒位在研究的茄属种中的存在/缺失情况，并使用 BAC-FISH 信号的顺序构建了系统发育树。与早期研究相比，本研究的数据为当前将物种分为茄属内不同组提供了支持；然而，也有一些值得注意的例外，例如 S. etuberosum（与番茄组关系较近，而与马铃薯组关系较远）和 S. lycopersicoides（与 S. pennellii 姐妹关系）的树位。这些明显的矛盾可能是由种间杂交事件和/或不完全谱系分选引起的。这种跨物种 BAC 作图技术为广泛物种的基因组组织、进化和系统发育关系提供了独特的信息。这些信息对于渐渗育种非常有帮助。
