Department of Pharmacology and Pharmacodynamics, medical University of Lublin, Poland.
Exp Clin Psychopharmacol. 2012 Aug;20(4):325-32. doi: 10.1037/a0028391. Epub 2012 Jun 11.
The purpose of the reported experiments was to examine the effects of imperatorin [9-(3-methylbut-2-enyloxy)-7H-furo[3,2-g]chromen-7-one], a bioactive furanocoumarin isolated from the fruits of Angelica archangelica (Angelica officinalis) on anxiety and memory-related behaviors of mice. Male Swiss mice were tested for anxiety and cognition, in the elevated plus maze test (EPM), using two different procedures. In the present experiments, imperatorin was administered acutely (at the doses of 5, 10, 20, 30, and 50 mg/kg); injections were made 15, 30, and 60 min before test (anxiety); 30 min before the first trial (memory acquisition); or immediately after the first trial (memory consolidation), as well as subchronically, twice a day for 6 days. On the seventh day, the mice were injected once with imperatorin (10 and 20 mg/kg, i.p.) 30 min before the test (anxiety) and 30 min before the first trial (memory acquisition), or immediately after the first trial (memory consolidation). We observed that imperatorin when administered acutely and repeatedly, at the doses of 10 and 20 mg/kg, exerted an anxiolytic effect on mice tested 30 min after the injection measured in the EPM test. By contrast, no such effect was observed after the acute administration of imperatorin at the doses of 5, 30 and 50 mg/kg. Moreover, other observations carried out 15 and 60 min after a single injection of the drug did not reveal any effect of imperatorin on anxiety behavior in the EPM test. Furthermore, acute and repeated administration of imperatorin (10 and 20 mg/kg) improved different stages of memory processes (both acquisition and consolidation) in a modified EPM test (mEPM). The results of our research suggest imperatorin to be an interesting therapeutical option in disorders with high anxiety levels and memory impairment.
本研究旨在探讨白芷素（9-（3-甲基-2-丁烯基氧基）-7H-呋喃[3,2-g]色烯-7-酮，一种从白芷（Angelica officinalis）果实中分离出的生物活性呋喃香豆素）对小鼠焦虑和记忆相关行为的影响。雄性瑞士小鼠在高架十字迷宫测试（EPM）中，通过两种不同的程序进行焦虑和认知测试。在本实验中，白芷素急性给药（剂量为 5、10、20、30 和 50mg/kg）；在测试前 15、30 和 60 分钟（焦虑）；在第一次试验前 30 分钟（记忆获得）；或在第一次试验后立即（记忆巩固）进行注射，以及亚慢性，每天两次，共 6 天。在第 7 天，小鼠在测试前 30 分钟（焦虑）和第一次试验前 30 分钟（记忆获得）或第一次试验后立即（记忆巩固），分别以 10 和 20mg/kg 腹腔注射一次白芷素。结果发现，当以 10 和 20mg/kg 的剂量急性和重复给药时，白芷素对注射后 30 分钟进行 EPM 测试的小鼠表现出抗焦虑作用。相比之下，当以 5、30 和 50mg/kg 的剂量急性给药时，未观察到这种作用。此外，单次注射药物后 15 和 60 分钟进行的其他观察未显示白芷素对 EPM 测试中焦虑行为的任何影响。此外，白芷素（10 和 20mg/kg）的急性和重复给药改善了改良 EPM 测试（mEPM）中记忆过程的不同阶段（获得和巩固）。我们的研究结果表明，白芷素是一种具有高焦虑水平和记忆障碍的疾病的有趣治疗选择。
