Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
Acta Biochim Biophys Sin (Shanghai). 2012 Aug;44(8):660-8. doi: 10.1093/abbs/gms045. Epub 2012 Jun 11.
Neural precursor cells play important roles in the neocortical development, but the mechanisms of neural progenitor proliferation, neuronal differentiation, and migration, as well as patterning are still unclear. Sox11, one of SoxC family members, has been reported to be essential for embryonic and adult neurogenesis. But there is no report about the roles of Sox11 in corticogenesis. In order to investigate Sox11 function during cortical development, loss of function experiment was performed in this study. Knockdown of Sox11 by Sox11 siRNA constructs resulted in a diminished neuronal differentiation, but enhanced proliferation of intermediate progenitors. Accompanied with the high expression of Sox11 in the postmitotic neurons, but low expression of Sox11 in the dividing neural progenitors, all the observations indicate that Sox11 induces neuronal differentiation during the neocortical development.
神经前体细胞在大脑皮质发育中发挥着重要作用，但神经祖细胞的增殖、神经元分化和迁移以及模式形成的机制尚不清楚。SoxC 家族成员之一 Sox11 已被报道对胚胎和成体神经发生至关重要。但目前尚无 Sox11 在皮质发生中的作用的报道。为了研究 Sox11 在皮质发育过程中的作用，本研究进行了功能丧失实验。Sox11 siRNA 构建物敲低 Sox11 导致中间祖细胞的神经元分化减少，但增殖增强。伴随着 Sox11 在有丝分裂后神经元中的高表达和 Sox11 在分裂神经祖细胞中的低表达，所有观察结果表明 Sox11 在大脑皮质发育过程中诱导神经元分化。
