Rimet O, Chauvet M, Bourdeaux M, Briand C, Sastre B
Laboratoire de Physique Pharmaceutique, Marseille, France.
Cancer Biochem Biophys. 1990 Jul;11(3):239-45.
As DHFR is the main molecular target of MTX, a widely used anticancer drug, its level in human tissues is likely to be one of the factors determining tissue sensitivity towards this drug. Forty-one biopsies were analyzed for their DHFR activity by a convenient spectrofluorometric assay developed in our laboratory; this sensitive method proved to be suitable for measurements in very small human samples. Statistical analysis of the results showed that (i) DHFR activity is not an index of tumorogenicity, at least in the cases studied, (ii) tumorous extracts contain modulators of DHFR activity.
由于二氢叶酸还原酶（DHFR）是广泛使用的抗癌药物甲氨蝶呤（MTX）的主要分子靶点，其在人体组织中的水平可能是决定组织对该药物敏感性的因素之一。通过我们实验室开发的一种便捷的荧光分光光度法，对41份活检样本的DHFR活性进行了分析；这种灵敏的方法被证明适用于对非常小的人体样本进行测量。结果的统计分析表明：（i）至少在所研究的病例中，DHFR活性不是肿瘤发生的指标；（ii）肿瘤提取物含有DHFR活性调节剂。
