Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore.
Proc Natl Acad Sci U S A. 2012 Jun 26;109(26):10214-7. doi: 10.1073/pnas.1200817109. Epub 2012 Jun 11.
Fluorescent small molecules have become indispensable tools for biomedical research along with the rapidly developing optical imaging technology. We report here a neural stem cell specific boron-dipyrromethane (BODIPY) derivative compound of designation red 3 (CDr3), developed through a high throughput/content screening of in-house generated diversity oriented fluorescence library in stem cells at different developmental stages. This novel compound specifically detects living neural stem cells of both human and mouse origin. Furthermore, we identified its binding target by proteomic analysis as fatty acid binding protein 7 (FABP7), also known as brain lipid binding protein) which is highly expressed in neural stem cells and localized in the cytoplasm. CDr3 will be a valuable chemical tool in the study and applications of neural stem cells.
荧光小分子与快速发展的光学成像技术一起，已成为生物医学研究中不可或缺的工具。我们在此报告一种新的神经干细胞特异性硼二吡咯甲烷（BODIPY）衍生物，命名为红色 3（CDr3），它是通过对不同发育阶段的干细胞中高通量/高含量的荧光库进行定向筛选而开发的。该新型化合物可特异性检测人源和鼠源的活神经干细胞。此外，通过蛋白质组学分析，我们将其结合靶标鉴定为脂肪酸结合蛋白 7（FABP7），也称为脑脂质结合蛋白，其在神经干细胞中高表达，并定位于细胞质中。CDr3 将成为神经干细胞研究和应用的有价值的化学工具。
