Tiwari Manjul
Department of Oral Pathology and Microbiology, School of Dental Sciences, Sharda University, Greater Noida, Uttar Pradesh, India.
J Nat Sci Biol Med. 2012 Jan;3(1):12-8. doi: 10.4103/0976-9668.95935.
DNA microarray technology has become a powerful tool in the arsenal of the molecular biologist. Capitalizing on high-precision robotics and the wealth of DNA sequences annotated from the genomes of a large number of organisms, the manufacture of microarrays is now possible for the average academic laboratory with the funds and motivation. Microarray production requires attention to both biological and physical resources, including DNA libraries, robotics, and qualified personnel. Although the fabrication of microarrays is a very labor-intensive process, production of quality microarrays individually tailored on a project-by-project basis will help researchers shed light on future scientific questions.
DNA微阵列技术已成为分子生物学家武器库中的一项强大工具。借助高精度机器人技术以及从大量生物体基因组中注释出的丰富DNA序列,现在资金充足且有积极性的普通学术实验室也能够制造微阵列。微阵列生产需要关注生物和物理资源,包括DNA文库、机器人技术和合格人员。尽管微阵列的制造是一个劳动强度很大的过程,但根据每个项目单独定制高质量微阵列将有助于研究人员阐明未来的科学问题。