Welsh School of Pharmacy, Cardiff University, CF10 3NB, UK.
Int J Pharm. 2012 Sep 15;434(1-2):399-405. doi: 10.1016/j.ijpharm.2012.06.008. Epub 2012 Jun 9.
The current work examined the effect of fish oil (FO) and betamethasone dipropionate (BD) on the growth of immortalized HaCaT keratinocytes. HaCaT cells were grown and treated with FO and/or BD, and proliferation determined using the MTT method. The cells were further probed by immunocytochemistry (ICC) techniques for apoptosis using Cleaved Caspase-3 Asp175, and inflammatory processes using cyclooxygenase-2 (COX-2). The addition of FO increased the inhibition of HaCaT cells by 27.2%, from 43.15% to 70.35% compared to BD alone (p 0.034). FO alone appeared to induce expression of Asp175 and the effect was greater in combination with BD. The net effect, however, were less than BD alone. Similar observations were seen with regards to COX-2 inhibition. The added benefits of FO to the effect of BD on the inhibition of cell growth, induction of apoptosis and inhibition of inflammation have now been demonstrated on a cellular level. Each of these activities supports beneficial effects in hyperproliferative skin disorders, such as psoriasis.
本研究旨在探讨鱼油（FO）和倍他米松二丙酸酯（BD）对永生化 HaCaT 角质形成细胞生长的影响。将 HaCaT 细胞进行培养并分别用 FO 和/或 BD 处理，通过 MTT 法测定细胞增殖。采用免疫细胞化学（ICC）技术检测凋亡过程中 Cleaved Caspase-3 Asp175 的表达，以及炎症过程中环氧化酶-2（COX-2）的表达。与单独使用 BD 相比，FO 可使 HaCaT 细胞的抑制率增加 27.2%，从 43.15%增加至 70.35%（p=0.034）。FO 单独使用似乎会诱导 Asp175 的表达，与 BD 联合使用的效果更大。然而，总的效果仍不及单独使用 BD。在 COX-2 抑制方面也观察到了类似的结果。FO 可增强 BD 对细胞生长抑制、诱导凋亡和抑制炎症的作用，这在细胞水平上已得到证实。这些活性均支持 FO 在治疗过度增殖性皮肤病（如银屑病）方面的有益作用。
