Cardiff University, UK.
Inflamm Res. 2010 Jan;59(1):23-30. doi: 10.1007/s00011-009-0065-z. Epub 2009 Jul 31.
To probe ex vivo the influence of fish oil (FO) on the topical delivery and anti-inflammatory properties of betamethasone dipropionate (BD).
Freshly excised porcine ear skin.
Ointment formulations containing BD + salicylic acid (SA), BD + SA + FO, or base as control, applied to the skin mounted in Franz cells.
Comparative depth profiling; skin probed by immunohistochemistry for cyclooxygenase-2 (COX-2) and by ELISA for prostaglandin E2 (PGE2).
More BD was obtained in the first 30 layers and the remaining epidermis with BD + SA. However, more penetrants were recovered from the remaining skin treated with BD + SA + FO. Although BD + SA reduced COX-2 expression within the epidermis, greater reduction was observed with BD + SA + FO as indicated by reduced COX-2 expression. FO alone had a comparable effect on the expression of COX-2. Modulation of PGE2 production also supported the anti-inflammatory properties of fish oil, reducing PGE2 levels by an amount comparable to the reduction by BD. Combining FO and BD, however, did not provide the anticipated potentiation effect.
Fish oil enhanced the delivery of BD and SA across skin. Addition of fish oil also enhanced the anti-inflammatory activity of BD, attributed to increased amounts of BD present in the skin and/or the intrinsic anti-inflammatory activity of fish oil.
在体外用鱼油（FO）探究倍他米松二丙酸酯（BD）的局部递药和抗炎特性。
新鲜猪耳朵皮肤。
将含有 BD + 水杨酸（SA）、BD + SA + FO 或作为对照的基础软膏制剂应用于安装在 Franz 细胞上的皮肤。
比较深度剖析；通过免疫组织化学探测皮肤中环氧化酶-2（COX-2），通过 ELISA 探测前列腺素 E2（PGE2）。
BD + SA 可在前 30 层和剩余表皮中获得更多 BD，但 BD + SA + FO 处理的剩余皮肤中可回收更多渗透物。尽管 BD + SA 降低了表皮内 COX-2 的表达，但与 BD + SA + FO 相比，观察到 COX-2 的表达减少更多。FO 单独对 COX-2 的表达也具有类似的作用。PGE2 产生的调节也支持鱼油的抗炎特性，其降低 PGE2 水平的量与 BD 相当。然而，将 FO 和 BD 结合使用并没有提供预期的增效作用。
鱼油增强了 BD 和 SA 在皮肤中的传递。添加鱼油还增强了 BD 的抗炎活性，归因于皮肤中存在更多的 BD 和/或鱼油的内在抗炎活性。
