Khor H, Rajendra R, Gopalakrishnan M, Tee E S
Department of Biochemistry, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Malays J Nutr. 1998 Dec;4(1):81-90.
Pure olive oil triglycerides (POLO), free from all unsaponifiable matter, were isolated from Virgin Spanish olive oil (COLO) by alumina-charcoal column chromatography. COLO and POLO were used as sources of dietary fat in two animal studies. The responses of serum and liver lipids to the two types of dietary fat were examined. Our results show that animals fed POLO-diet gave somewhat higher serum total and LDL cholesterol levels as compared to those on COLO-diet. The increase in serum cholesterol level is followed by a parallel increase in liver cholesterol content. These results indicate that the hypocholesterolemic effect of olive oil was partly due to the presence of the unsaponifiable matter. Supplement of the POLO-diet separately with a-tocopherol and squalene resulted in serum lipid responses similar to that observed with the COLO-diet. The serum and liver triglyceride levels are not affected by the removal of unsaponifiable components but addition of a--T and squalene to the POLO-diet appeared to lower both the cholesterol and triglyceride levels in the serum but increased only the liver cholesterol content. These results show that the unsaponifiable components modulate the hypocholesterolemic effect of olive oil.
通过氧化铝 - 活性炭柱色谱法从西班牙初榨橄榄油（COLO）中分离出不含所有不皂化物的纯橄榄油甘油三酯（POLO）。在两项动物研究中，COLO和POLO被用作膳食脂肪来源。研究了血清和肝脏脂质对这两种膳食脂肪的反应。我们的结果表明，与食用COLO饮食的动物相比，食用POLO饮食的动物血清总胆固醇和低密度脂蛋白胆固醇水平略高。血清胆固醇水平升高后，肝脏胆固醇含量也随之平行升高。这些结果表明，橄榄油的降胆固醇作用部分归因于不皂化物的存在。在POLO饮食中分别补充α-生育酚和角鲨烯，其血清脂质反应与COLO饮食相似。血清和肝脏甘油三酯水平不受不皂化成分去除的影响，但在POLO饮食中添加α-T和角鲨烯似乎可降低血清中的胆固醇和甘油三酯水平，但仅增加肝脏胆固醇含量。这些结果表明，不皂化成分调节了橄榄油的降胆固醇作用。
