Department of Soil Science, University of Saskatchewan, Saskatoon, Canada, S7N 5A8.
Mycorrhiza. 2013 Jan;23(1):45-59. doi: 10.1007/s00572-012-0448-9. Epub 2012 Jun 13.
The use of commercial inoculants containing non-resident arbuscular mycorrhizal fungi (AMF) is an emerging technology in field crop production in Canada. The objective of this study was to assess the impact of AMF inoculants containing either a single species (Glomus irregulare) or mixed species (G. irregulare, Glomus mosseae, and Glomus clarum) on AMF root colonization and consequent plant growth parameters of field pea grown using pot cultures. Field pea was grown in both sterilized and non-sterile (i.e., natural) field-collected soil containing resident AMF and received three inoculation treatments: uninoculated control, G. irregulare only, and a mixture of AMF species of G. irregulare, G. mosseae, and G. clarum. After 42 days, the AMF community assembled in field pea roots was assessed by cloning and sequencing analysis on the LSU-ITS-SSU rDNA gene, together with a microscopic assessment of colonization, biomass production, nutrient uptake, and N(2) fixation. The identity of AMF inoculants had a significant effect on field pea performance. The mixed species AMF inoculant performed better than the single species G. irregulare alone by promoting mycorrhizal colonization, field pea biomass, N and P uptake, and N(2) fixation and did not result in a significant compositional change of the AMF community that subsequently assembled in field pea roots. In contrast, the single species G. irregulare inoculant did not significantly enhance field pea biomass, N and P uptake, and N(2) fixation, although a significant compositional change of the subsequent AMF community was observed. No significant interactions affecting these measurements were detected between the resident AMF and the introduced AMF inoculants. The observation that the mixed species AMF inoculant promoted plant growth parameters without necessarily affecting the subsequent AMF community may have important implications regarding the use of non-resident AMF inoculants in agricultural production.
商业接种剂中使用非本地丛枝菌根真菌（AMF）是加拿大田间作物生产中的一项新兴技术。本研究的目的是评估含有单一物种（G. irregular）或混合物种（G. irregular、G. mosseae 和 G. clarum）的 AMF 接种剂对豌豆根定植的影响，以及对生长在经灭菌和未灭菌（即自然）田间采集土壤中的豌豆生长参数的影响，土壤中含有本地 AMF。豌豆在经灭菌和未灭菌（即自然）田间采集土壤中生长，并接受三种接种处理：未接种对照、仅接种 G. irregular 和 AMF 混合接种（G. irregular、G. mosseae 和 G. clarum）。42 天后，通过克隆和测序分析 LSU-ITS-SSU rDNA 基因评估豌豆根中组装的 AMF 群落，同时进行定植、生物量生产、养分吸收和 N2 固定的显微镜评估。AMF 接种剂的身份对豌豆的表现有显著影响。与单独使用单一物种 G. irregular 相比，混合物种 AMF 接种剂通过促进丛枝菌根定植、豌豆生物量、N 和 P 吸收以及 N2 固定的效果更好，且不会导致随后在豌豆根中组装的 AMF 群落发生显著的组成变化。相比之下，虽然观察到随后组装的 AMF 群落的组成发生了显著变化，但单一物种 G. irregular 接种剂并未显著提高豌豆生物量、N 和 P 吸收以及 N2 固定。在这些测量中，没有检测到本地 AMF 和引入的 AMF 接种剂之间存在显著的相互作用。观察到混合物种 AMF 接种剂在不影响随后的 AMF 群落的情况下促进植物生长参数，这可能对农业生产中使用非本地 AMF 接种剂具有重要意义。
