Smith Laura A, Ma Peter X
Department of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, MI, USA.
Methods Mol Biol. 2012;868:125-34. doi: 10.1007/978-1-61779-764-4_8.
Nano-fibrous scaffolding mimics aspects of the extracellular matrix to improve cell function and tissue formation. Although several methods exist to fabricate nano-fibrous scaffolds, the combination of phase separation with reverse solid freeform fabrication (SFF) allows for scaffolds with features at three different orders of magnitude to be formed, which is not easily achieved with other nano-fiber fabrication methods. This technique allows for the external shape and internal pore structure to be precisely controlled in an easily repeatable manner, while the nano-fibrous wall architecture facilitates cellular attachment, proliferation, and differentiation of the cells. In this chapter, we examine the fabrication of computer-designed nano-fibrous scaffolds utilizing thermally induced phase separation and reverse SFF, and the benefits of such scaffolds over more traditional tissue engineering scaffolds on cellular function and tissue regeneration.
纳米纤维支架模仿细胞外基质的某些方面以改善细胞功能和组织形成。虽然存在几种制造纳米纤维支架的方法，但相分离与反向实体自由成型制造（SFF）相结合能够形成具有三种不同数量级特征的支架，这是其他纳米纤维制造方法不易实现的。该技术能够以易于重复的方式精确控制外部形状和内部孔隙结构，而纳米纤维壁结构有利于细胞附着、增殖和分化。在本章中，我们研究利用热致相分离和反向SFF制造计算机设计的纳米纤维支架，以及此类支架相对于更传统的组织工程支架在细胞功能和组织再生方面的优势。
