Cozzi A, Santambrogio P, Levi S, Arosio P
Department of Biomedical Science and Technology, University of Milano, San Raffaele Institute, Italy.
FEBS Lett. 1990 Dec 17;277(1-2):119-22. doi: 10.1016/0014-5793(90)80823-2.
Three recombinant human apoferritin variants were added to ferrous iron and the amount of lipid peroxidation produced by hydrogen peroxide was studied. The H-apoferritin had the strongest inhibitory effect on lipid peroxidation, probably due to its ferroxidase activity. The L-apoferritin inhibited lipid peroxidation slowly and only at neutral pH. The H-mutant 91, deleted of the last 22 C-terminal amino acids, and which is not able to form an iron core, had minimal effects on iron lipid peroxidation. It was concluded that both ferro-oxidase and iron mineralization activities are necessary for ferritin iron detoxifying action.
将三种重组人脱铁铁蛋白变体添加到亚铁中，研究了过氧化氢产生的脂质过氧化量。H-脱铁铁蛋白对脂质过氧化具有最强的抑制作用，这可能归因于其亚铁氧化酶活性。L-脱铁铁蛋白对脂质过氧化的抑制作用缓慢，且仅在中性pH值时起作用。H-突变体91缺失了最后22个C末端氨基酸，无法形成铁芯，对铁脂质过氧化的影响最小。得出的结论是，亚铁氧化酶和铁矿化活性对于铁蛋白的铁解毒作用都是必需的。
