Institute of Biological Sciences/Discipline of Pharmacology.
Clin Med Insights Oncol. 2012;6:233-42. doi: 10.4137/CMO.S9518. Epub 2012 May 28.
To verify if patients with cervical neoplasia produce mediators that reduce leukocyte function.
Control neutrophils incubated with normal serum or serum from pre-invasive or invasive neoplasia patients were assayed for chemotaxis. Mediators were assayed in serum and in leukocyte supernatants. Experiments were also performed in random patients after surgery.
Neutrophils incubated with patient sera, but not normal sera, failed to migrate towards the chemoattractants. In invasive neoplasia compared to controls, IL-6 and IL-8, and IL-10 and TNF-α were elevated in serum and in neutrophil supernatants, respectively. Nitrite levels were elevated in mononuclear cell supernatants from patients than controls. After surgery, serum cytokine levels were reduced, mainly in pre-invasive patients. Neutrophils treated with serum from pre-invasive patients undergone surgery had restored migration.
Patients with cervical neoplasia produce mediators, predominantly induced by tumor cells, able to impair the inflammatory response at very early stages of disease.
验证患有宫颈癌的患者是否产生可降低白细胞功能的介质。
用正常血清或来自癌前或侵袭性肿瘤患者的血清孵育对照中性粒细胞，并检测其趋化性。在血清和白细胞上清液中检测介质。还在手术后随机患者中进行了实验。
与正常血清相比，用患者血清孵育的中性粒细胞不能向趋化因子迁移。与对照组相比，在侵袭性肿瘤中，IL-6 和 IL-8 以及 IL-10 和 TNF-α 分别在血清和中性粒细胞上清液中升高。单核细胞上清液中的亚硝酸盐水平也高于对照组。手术后，血清细胞因子水平降低，主要在癌前病变患者中。用来自接受手术的癌前病变患者的血清处理的中性粒细胞的迁移能力得到恢复。
宫颈癌患者产生的介质主要由肿瘤细胞诱导，能够在疾病的早期阶段损害炎症反应。
