Reisman D
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
FEBS Lett. 1990 Dec 17;277(1-2):209-11. doi: 10.1016/0014-5793(90)80846-b.
The transcriptional enhancer of the Moloney Murine Leukemia virus (Mo-MuLV) is comprised of a 75-bp direct repeat, each of which contains multiple binding sites for transcription factors. The occupancy of these sites determines the tissue specificity of expression and disease tropism of the virus. The identification of proteins that bind to this enhancer is therefore required in order to understand the molecular basis of this viral-host interaction. Analysis of the nucleic acid sequence of the Mo-MuLV has identified 4 potential binding sites for the transcription factor NF-1. Evidence is presented using DNAase I protection analysis that NF-1 binds to these 4 sites within the enhancer. The potential role of NF-1 binding in tissue specific expression of Mo-MuLV is discussed.
莫洛尼鼠白血病病毒（Mo-MuLV）的转录增强子由一个75碱基对的直接重复序列组成，每个重复序列都包含多个转录因子结合位点。这些位点的占据情况决定了病毒表达的组织特异性和疾病嗜性。因此，为了理解这种病毒-宿主相互作用的分子基础，需要鉴定与该增强子结合的蛋白质。对Mo-MuLV核酸序列的分析确定了转录因子NF-1的4个潜在结合位点。通过DNA酶I保护分析提供的证据表明，NF-1与增强子内的这4个位点结合。讨论了NF-1结合在Mo-MuLV组织特异性表达中的潜在作用。
