Klaus S, Casteilla L, Hentz E, Dani C, Ricquier D
Centre de Recherche sur la Nutrition (CNRS), Meudon Bellevue, France.
Mol Cell Endocrinol. 1990 Oct 22;73(2-3):105-10. doi: 10.1016/0303-7207(90)90123-p.
The thyroid hormone 3,3',5-triiodothyronine (T3) plays an important role in the differentiation of adipocytes, as well as in the thermogenic activity of brown adipose tissue. Recently a T3 binding protein (T3BP), which is associated with plasma membranes, has been isolated and cloned from liver. It proved to be homologous to the multifunctional enzyme protein disulfide isomerase (PDI), which is involved in posttranslational modifications of secretory proteins. In this study we investigated the T3BP/PDI mRNA expression in white and brown adipose tissue of rat and bovine, as well as in several rodent adipose cell lines at various states of differentiation. T3BP/PDI mRNA expression was found in white and brown adipose tissue, as well as in preadipocytes and adipocytes at all states of differentiation. Comparison to other tissues (liver, kidney, heart, brain) revealed that its expression was highest in white fat. No modulation of T3BP/PDI mRNA corresponding to different adaptational or developmental situations could be detected in adipose tissue.
甲状腺激素3,3',5-三碘甲状腺原氨酸（T3）在脂肪细胞分化以及棕色脂肪组织的产热活性中发挥着重要作用。最近，一种与质膜相关的T3结合蛋白（T3BP）已从肝脏中分离并克隆出来。事实证明，它与参与分泌蛋白翻译后修饰的多功能酶蛋白二硫键异构酶（PDI）同源。在本研究中，我们调查了大鼠和牛的白色及棕色脂肪组织以及几种处于不同分化状态的啮齿动物脂肪细胞系中T3BP/PDI mRNA的表达情况。在白色和棕色脂肪组织以及处于所有分化状态的前脂肪细胞和脂肪细胞中均发现了T3BP/PDI mRNA的表达。与其他组织（肝脏、肾脏、心脏、大脑）相比，发现其在白色脂肪中的表达最高。在脂肪组织中未检测到与不同适应或发育情况相对应的T3BP/PDI mRNA的调节。
