Qureshi I Z, White S J
Department of Biomedical Science, Sheffield University, Western Bank Sheffield, Sheffield, S10 2TN, UK.
Anat Histol Embryol. 2013 Feb;42(1):72-8. doi: 10.1111/j.1439-0264.2012.01168.x. Epub 2012 Jun 14.
The receptors for sulphonylurea (SURs) are known to be expressed in the mouse kidney, but their expression in the ureter is undefined. Owing to the physiological and pharmacological significance of SURs, the localization of SUR in ureters of adult mice and rats was investigated through immunohistochemistry. Animals were perfused transcardially with 4% paraformaldehyde and tissues were processed for immunohistochemistry using polyclonal antisera against SUR2A and SUR2B (SUR1) receptor proteins. Sections were incubated with primary and secondary antisera and developed with aminoethylcarbazole as a chromogen. A differentiated localized staining pattern of SUR proteins in rat and mouse ureters is demonstrated. In the mouse, immunoreactivity of SUR2A was predominantly confined to the cytoplasmic portion of epithelial cells and blood vessels, with comparatively low-level staining found in smooth muscle. In contrast, SUR2B (SUR1) immunoreactivity was absent in mouse ureters. In rats, SUR2A immunoreactivity was localized only in the blood vessels, while SUR2B (SUR1) immunoreactivity was localized in the epithelial cell cytoplasm. Tissue specificity of SUR is demonstrated in the two species of rodents and suggests a role of SUR proteins in urinary metabolism pertaining possibly to salt handling and maintenance of the smooth muscle tone.
已知磺脲类受体（SURs）在小鼠肾脏中表达，但其在输尿管中的表达尚不明确。鉴于SURs的生理和药理意义，通过免疫组织化学研究了成年小鼠和大鼠输尿管中SUR的定位。动物经心脏灌注4%多聚甲醛，组织用针对SUR2A和SUR2B（SUR1）受体蛋白的多克隆抗血清进行免疫组织化学处理。切片与一抗和二抗孵育，并用氨基乙基咔唑作为显色剂显色。结果显示大鼠和小鼠输尿管中SUR蛋白存在差异定位的染色模式。在小鼠中，SUR2A的免疫反应主要局限于上皮细胞和血管的细胞质部分，平滑肌中的染色水平相对较低。相比之下，小鼠输尿管中不存在SUR2B（SUR1）免疫反应。在大鼠中，SUR2A免疫反应仅定位于血管，而SUR2B（SUR1）免疫反应定位于上皮细胞细胞质。在这两种啮齿动物中均显示了SUR的组织特异性，提示SUR蛋白在尿液代谢中可能与盐处理和平滑肌张力维持有关。
