Shukla G, Asnani P J, Bhusnurmath S R, Vinayak V K
Department of Experimental Medicine, Postgraduate Institute of Medical Education & Research, Chandigarh.
Indian J Med Res. 1990 Sep;91:372-8.
Pre-pregnancy immunization of Swiss albino mice with merozoite antigen of P. berghei entrapped in multilamellar phosphatidyl choline liposomes resulted in (i) increased prepatent period, (ii) either no or low parasitaemic levels, (iii) reduced mortality, and (iv) normal foetal and placental development, upon challenge with P. berghei on 13th gestational day. The unimmunized animals which received either phosphate buffered saline or empty multilamellar phosphatidyl choline liposomes before pregnancy developed high parasitaemic and 30-40 per cent animals died before parturition while 60-70 per cent unimmunized animals revealed foetal abnormalities such as low body weight and larger spleen size. Placentae of unprotected animals had hyperplasia of trophoblastic membrane and plugging of placental sinusoids with parasitized erythrocytes and malarial pigments. The data suggest that prior immunization of animals with merozoite antigen entrapped in multilamellar phosphatidyl choline liposomes could abrogate the ill effects induced by malaria infection under the stress of pregnancy.
用包裹于多层磷脂酰胆碱脂质体中的伯氏疟原虫子孢子抗原对瑞士白化小鼠进行孕前免疫，结果显示，在妊娠第13天用伯氏疟原虫攻击后，（i）潜伏期延长，（ii）血中疟原虫水平无或较低，（iii）死亡率降低，（iv）胎儿和胎盘发育正常。未免疫的动物在怀孕前接受磷酸盐缓冲盐水或空的多层磷脂酰胆碱脂质体，血中疟原虫水平较高，30%-40%的动物在分娩前死亡，而60%-70%未免疫的动物出现胎儿异常，如体重低和脾脏肿大。未受保护动物的胎盘有滋养层膜增生，胎盘血窦被寄生红细胞和疟色素堵塞。数据表明，用包裹于多层磷脂酰胆碱脂质体中的子孢子抗原预先免疫动物，可以消除妊娠应激下疟疾感染所诱导的不良影响。
