ChiroQuest Chiral Technologies Development Ltd., Rumbach S. u. 7, H-1075 Budapest, Hungary.
J Pharm Biomed Anal. 2012 Nov;70:71-6. doi: 10.1016/j.jpba.2012.05.023. Epub 2012 May 30.
Three β-cyclodextrin-based chiral stationary phases were developed applying novel bonding chemistry. The separation performances of β-cyclodextrin, (R,S)-2-hydroxypropyl-β-cyclodextrin, and permethyl-β-cyclodextrin-based CSPs were compared in the resolution of structurally divergent analytes, such as coumarins, dansyl amino acids, and propionic acid derivatives. Separations were carried out in reversed phase mode applying 0.1% triethylammonium phosphate (pH 3.5)/MeOH mobile phase systems in different compositions. Of the three novel CSPs the permethyl-β-cyclodextrin bonded phase proved to be the most effective one for the enantioseparation of investigated analytes.
开发了三种基于β-环糊精的手性固定相，应用了新颖的键合化学。在对结构差异较大的分析物（如香豆素、丹磺酰基氨基酸和丙酸衍生物）进行拆分时，比较了β-环糊精、（R，S）-2-羟丙基-β-环糊精和全甲基化-β-环糊精基 CSP 的分离性能。在反相模式下，采用不同组成的 0.1%三乙铵磷酸盐（pH 3.5）/甲醇流动相系统进行分离。在这三种新型 CSP 中，全甲基化-β-环糊精键合相被证明是拆分所研究分析物最有效的手性固定相。
