State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Arch Insect Biochem Physiol. 2012 Aug;80(3):140-50. doi: 10.1002/arch.21035. Epub 2012 Jun 13.
The Asian corn borer, Ostrinia furnacalis, is a serious pest of corn, sorghum, and cotton in China and other Asian countries. The present study is the first attempt to establish the transgenic line in O. furnacalis using a piggyBac transposon, which will shed light on the future genetic control of O. furnacalis. A piggyBac vector pBac[A3EGFP] was constructed to express enhanced green fluorescence protein (EGFP)under the control of Bombyx mori actin3 promoter. Transient EGFP expression was detected 48 h after preblastodermic microinjection of pBac[A3EGFP] and the excision assay showed the transgenic vector was precisely excised. In G1 animals, PCR (polymerase chain reaction)-based investigations revealed that the exogenous gene had been introduced into O. furnacalis genome and expressed at the transcriptional level. Western blot analysis showed EGFP expression at the protein level, indicating the heritability of the transgene.
亚洲玉米螟，Ostrinia furnacalis，是中国和其他亚洲国家玉米、高粱和棉花的严重害虫。本研究首次尝试使用 piggyBac 转座子在 O. furnacalis 中建立转基因系，这将为未来的 O. furnacalis 遗传控制提供启示。构建了一个 piggyBac 载体 pBac[A3EGFP]，用于在家蚕肌动蛋白 3 启动子的控制下表达增强型绿色荧光蛋白（EGFP）。在原肠胚期微注射 pBac[A3EGFP]后 48 小时检测到瞬时 EGFP 表达，并且切除测定表明转基因载体被精确切除。在 G1 代动物中，基于 PCR（聚合酶链反应）的研究表明，外源基因已被导入 O. furnacalis 基因组并在转录水平表达。Western blot 分析显示在蛋白质水平上表达 EGFP，表明转基因的遗传性。
