Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
Ultrasound Med Biol. 2012 Aug;38(8):1383-90. doi: 10.1016/j.ultrasmedbio.2012.04.002. Epub 2012 Jun 12.
Vascular smooth muscle cells (VSMCs) play an important role in the good performance of the vasculature. To study the surface, intracellular structure and elasticity of VSMCs, atomic force acoustic microscope (AFAM) was used for imaging VSMCs from A7r5 rat aorta arteries. The topography images of VSMCs were obtained in contact mode and the acoustic images were obtained by AFAM in sample vibration mode. Then, the force curve measurement derived using Young's modulus of the interested areas was used for evaluating elasticity properties. The acoustic images were found in higher resolution with more information than the topography images. The force curves showed the difference in Young's modulus of the different parts of VSMC. These findings demonstrate that AFAM is useful for displaying the surface, structure and elasticity property of VSMCs clearly, with short scanning time, negligible harm or damage to cell and nanometer-level resolution.
血管平滑肌细胞（VSMCs）在血管的良好功能中起着重要作用。为了研究 VSMCs 的表面、细胞内结构和弹性，使用原子力声显微镜（AFAM）对来自 A7r5 大鼠主动脉的 VSMCs 进行成像。以接触模式获得 VSMCs 的形貌图像，并以 AFAM 的样品振动模式获得声图像。然后，使用感兴趣区域的杨氏模量推导力曲线测量值，以评估弹性性质。声图像的分辨率高于形貌图像，且包含更多信息。力曲线显示了 VSMC 不同部位杨氏模量的差异。这些发现表明，AFAM 可用于清晰显示 VSMCs 的表面、结构和弹性特性，具有短的扫描时间、对细胞几乎没有伤害或损坏以及纳米级分辨率。
