State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei 430072, China.
J Biosci Bioeng. 2012 Oct;114(4):405-10. doi: 10.1016/j.jbiosc.2012.05.007. Epub 2012 Jun 13.
To evaluate the potential of the green agla Chlorella zofingiensis as a feedstock for biodiesel production, the effects of nitrogen and phosphate on lipid accumulation and growth of C. zofingiensis were studied. The maximum specific growth rate (μ(max)) reached 2.15 day⁻¹ when the concentration of NaNO₃ and K₂HPO₄·3H₂O was 1.0 g L⁻¹ and 0.01 g L⁻¹, respectively. The lipid contents of C. zofingiensis grown in media deficient of nitrogen (65.1%) or phosphate (44.7%) were both higher than that obtained from cells grown in full medium (33.5%). The highest lipid productivity (87.1 mg L⁻¹ day⁻¹) was also obtained from cells grown in nitrogen deficient media, indicating nitrogen deficiency was more effective than phosphate deficiency for inducing lipid accumulation in C. zofingiensis. In addition, the feasibility of cultivating the alga in 60 L flat plate photobioreactors and 10 L bottles outdoors for biodiesel was also tested. It was found that C. zofingiensis could adapt to fluctuating temperatures and irradiance of outdoors and the highest μ(max) and lipid productivity could reach 0.362 day⁻¹ and 26.6 mg L⁻¹ day⁻¹ outdoors, respectively. The lipid production potential of C. zofingiensis is projected to be 31.1 kg ha⁻¹ day⁻¹ in outdoor culture. These results suggested that C. zofingiensis is a promising organism for feedstock production of biofuel and can be used in scaled up culture outdoors.
为了评估绿色藻类栅藻（Chlorella zofingiensis）作为生物柴油生产原料的潜力，研究了氮和磷对栅藻脂质积累和生长的影响。当 NaNO₃ 和 K₂HPO₄·3H₂O 的浓度分别为 1.0 g/L 和 0.01 g/L 时，栅藻的最大比生长速率（μ(max)）达到 2.15 天⁻¹。在缺乏氮（65.1%）或磷（44.7%）的培养基中生长的栅藻的脂质含量均高于在完全培养基中生长的细胞（33.5%）。在氮缺乏的培养基中培养的细胞也获得了最高的脂质生产力（87.1 mg/L·天⁻¹），表明氮缺乏比磷缺乏更有效地诱导栅藻的脂质积累。此外，还测试了在 60 L 平板光生物反应器和 10 L 室外瓶中培养藻类以生产生物柴油的可行性。结果发现，栅藻能够适应室外温度和光照的波动，室外条件下的最高μ(max)和脂质生产力分别达到 0.362 天⁻¹和 26.6 mg/L·天⁻¹。栅藻的脂质生产潜力预计在室外培养条件下为 31.1 kg·ha⁻¹·天⁻¹。这些结果表明，栅藻是生物燃料原料生产的有前途的生物，可用于室外放大培养。
