Department of Chemistry, Faculty of Science, Razi University, Kermanshah, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2012 Oct;96:278-83. doi: 10.1016/j.saa.2012.05.045. Epub 2012 May 24.
The interaction of native calf thymus DNA with the drug levetiracetam (LV) has been investigated by absorption, emission, circular dichroism, melting temperature (T(m)) curves and viscosity studies. Spectrophotometric studies of the interaction of levetiracetam with DNA have shown that it can binds to CT-DNA and the DNA binding constant (K(b)=(4.9±0.2)×10(3) M(-1)) is comparable to groove binding drugs. Competitive fluorimetric studies with hoechst 33258 have shown that levetiracetam exhibit the ability to displace the DNA-bound hoechst 33258 indicating that it binds to DNA in strong competition with hoechst 33258 for the groove binding. Furthermore, the drug induces detectable changes in the CD spectrum of CT-DNA as well as changes in its viscosity. The values of melting temperature (T(m)) of DNA-methylene blue (MB) complex and LV-DNA-MB complex were determined. Finally, all of the experimental results prove that the minor groove binding must be predominant.
研究了天然小牛胸腺 DNA 与药物左乙拉西坦（LV）的相互作用，通过吸收、发射、圆二色性、熔点（T(m)) 曲线和粘度研究进行了研究。左乙拉西坦与 DNA 相互作用的分光光度研究表明，它可以与 CT-DNA 结合，DNA 结合常数（K(b)=（4.9±0.2)×10(3) M(-1)）与沟结合药物相当。与 Hoechst 33258 的竞争性荧光研究表明，左乙拉西坦表现出置换 DNA 结合的 Hoechst 33258 的能力，表明它与 Hoechst 33258 强烈竞争沟结合。此外，该药物诱导 CT-DNA 的 CD 光谱以及其粘度发生可检测的变化。测定了 DNA-亚甲蓝（MB）复合物和 LV-DNA-MB 复合物的熔点（T(m)) 值。最后，所有实验结果都证明，小沟结合必须占主导地位。
