小鼠乳腺肿瘤病毒的体外感染性测定

In vitro infectivity assay for mouse mammary tumor virus.

作者信息

Vacquier J P, Cardiff R D

出版信息

Proc Natl Acad Sci U S A. 1979 Aug;76(8):4117-21. doi: 10.1073/pnas.76.8.4117.

DOI:10.1073/pnas.76.8.4117

PMID:226995

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC383989/

Abstract

Studies of mouse mammary tumor virus (MMTV) have been impeded by the lack of an in vitro infectivity assay. We have developed a rapid, quantitative in vitro assay for MMTV infectivity based on the detection of positively staining foci by immunoperoxidase. This assay and a 50% end-point titration of MMTV infectivity gave identical virus titers. Infection of a rat hepatoma cell line, a feline kidney cell line, and a normal murine mammary gland cell line by virus from the mouse mammary tumor GR3A cell line was linear with respect to virus concentration. The infectious titers obtained in both homologous and heterologous cell lines were not significantly different, demonstrating a lack of host range specificity. Virus infectivity was inactivated by heating at 55 degrees C and by ultraviolet irradiation. Rabbit anti-MMTV serum neutralized the infectivity with a 50% neutralization end point of 1:5000. Applications of this assay to the study of the immunological, biological, and biochemical characteristics of MMTV are discussed.

摘要

由于缺乏体外感染性检测方法，小鼠乳腺肿瘤病毒（MMTV）的研究受到了阻碍。我们基于免疫过氧化物酶检测阳性染色灶，开发了一种快速、定量的MMTV体外感染性检测方法。该检测方法与MMTV感染性的50%终点滴定法得出的病毒滴度相同。来自小鼠乳腺肿瘤GR3A细胞系的病毒对大鼠肝癌细胞系、猫肾细胞系和正常小鼠乳腺细胞系的感染与病毒浓度呈线性关系。在同源和异源细胞系中获得的感染滴度没有显著差异，表明缺乏宿主范围特异性。病毒感染性在55℃加热和紫外线照射下会失活。兔抗MMTV血清中和感染性，50%中和终点为1:5000。本文讨论了该检测方法在MMTV免疫、生物学和生化特性研究中的应用。