Teramoto Y A, Puentes M J, Young L J, Cardiff R D
J Virol. 1974 Feb;13(2):411-8. doi: 10.1128/JVI.13.2.411-418.1974.
The polypeptide and glycoprotein compositions of the mouse mammary tumor virus virion from primary monolayer cultures of BALB/cfC3H mouse mammary tumor cells were studied by polyacrylamide gel electrophoresis by using internal and external labeling and Coomassie blue and periodic acid Schiff (PAS) staining. Twelve polypeptides were reproducibly resolved by the combined methods. Five major polypeptides were demonstrable with estimated molecular weights of 52,000, 36,000, 28,000, 14,000, and 10,000. Seven minor polypeptides were also consistently detected and had estimated molecular weights of 70,000, 60,000, 46,000, 38,000, 30,000, 22,000, and 17,000. Carbohydrate was associated with five of these polypeptides as measured by PAS stain or [(3)H] glucosamine labeling, or both. These glycoproteins had estimated molecular weights of 70,000, 60,000, 52,000, 36,000 and 10,000. The majority of the PAS stain and glucosamine was found in the 52,000 and 36,000 dalton peaks.
利用内部和外部标记以及考马斯亮蓝和过碘酸希夫（PAS）染色，通过聚丙烯酰胺凝胶电泳研究了来自BALB/cfC3H小鼠乳腺肿瘤细胞原代单层培养物的小鼠乳腺肿瘤病毒病毒粒子的多肽和糖蛋白组成。通过联合方法可重复地分离出12种多肽。可证明有5种主要多肽，估计分子量分别为52,000、36,000、28,000、14,000和10,000。还始终检测到7种次要多肽，估计分子量分别为70,000、60,000、46,000、38,000、30,000、22,000和17,000。通过PAS染色或[（3）H]葡糖胺标记或两者测量，碳水化合物与其中5种多肽相关。这些糖蛋白的估计分子量为70,000、60,000、52,000、36,000和10,000。大部分PAS染色和葡糖胺存在于52,000和36,000道尔顿的峰中。
