体内亚细胞成像：下一次绿色荧光蛋白革命。

Subcellular imaging in vivo: the next GFP revolution.

作者信息

Hoffman Robert M

机构信息

AntiCancer, Inc., Department of Surgery, University of California, San Diego, CA, USA.

出版信息

Methods Mol Biol. 2012;872:255-63. doi: 10.1007/978-1-61779-797-2_18.

DOI:10.1007/978-1-61779-797-2_18

PMID:22700417

Abstract

The use of fluorescent proteins to differentially label cancer cells in the nucleus and cytoplasm and high-powered imaging technology have been used to visualize the nuclear-cytoplasmic dynamics of cancer-cell in vivo. Nuclear-cytoplasmic dynamics have been imaged in cancer cells trafficking in both blood vessels and lymphatic vessels as well as during seeding on organs and interacting with stroma in the live animal. Fluorescent proteins have also been used to color code the phases of the cell cycle which can now be followed in vivo. This technology has furthered our understanding of the spread of cancer at the subcellular level. Fluorescent proteins thereby provide the basis for the new field of in vivo cell biology.

摘要

利用荧光蛋白对癌细胞的细胞核和细胞质进行差异标记，以及借助高分辨率成像技术，已实现对体内癌细胞核质动力学的可视化。在活体动物中，已对癌细胞在血管和淋巴管中的运输过程以及在器官上播种并与基质相互作用期间的核质动力学进行了成像。荧光蛋白还被用于对细胞周期的各个阶段进行颜色编码，现在可以在体内追踪这些阶段。这项技术加深了我们对癌症在亚细胞水平扩散的理解。荧光蛋白从而为体内细胞生物学这一新领域奠定了基础。