Department of Physiology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Poult Sci. 2012 Jul;91(7):1598-603. doi: 10.3382/ps.2011-02042.
Prebiotics are being used as an alternative to antibiotic growth promoters in poultry industry with a variable degree of success. This trial was conducted to investigate the effect of dietary mannan-oligosaccharide (MOS) supplementation on growth performance, selected colonic bacterial population, and immune response in maternally-isolated rock pigeons. The pigeons (n = 36) were randomly distributed in 4 treatment groups (n = 9 per group) with 3 replicates or pens (n = 3) in each group. Birds were fed either a corn/wheat-based basal diet (control group/CON) or the same diet supplemented with 0.1%, 0.2%, or 0.5% MOS for 35 d. On d 35, birds were killed to collect visceral organs, colonic contents, and serum. Colonic contents were used to enumerate selected microbiota and serum was used to detect antibody titer against the Newcastle disease virus. Cell-mediated immunity was determined by measuring the skin thickness following 2,4-dinitrochlorobenzene challenge. Results showed that supplementation did not affect the BW of birds. During wk 4, feed intake was significantly higher in the 0.2% (187.9 ± 0.86) and the 0.5% (186.4 ± 0.86) MOS-supplemented groups compared with the CON group (180.7 ± 0.86). Gizzard weights (with and without digesta) were significantly higher in the MOS 0.1% (10.67 ± 0.33 and 8.22 ± 0.26) and the MOS 0.2% (9.91 ± 0.33 and 7.94 ± 0.26) groups compared with the CON group (7.56 ± 0.33 and 6.25 ± 0.26). Small intestinal weight was significantly higher in the MOS 0.2% group (14.71 ± 0.56) compared with the CON group (9.56 ± 0.56). Lengths of small intestine (MOS 0.1% = 92.56 ± 1.69, MOS 0.2% = 90.79 ± 1.69, MOS 0.5% = 90.57 ± 1.69) in all the MOS-fed groups and large intestine in the 0.1% (3.50 ± 0.02) and the 0.5% (3.47 ± 0.02) MOS-fed groups were significantly higher than the CON group (small intestine = 77.63 ± 1.69, large intestine = 2.41 ± 0.02). Weights of heart, liver, pancreas, and large intestine remained unaffected. Feeding of MOS did not influence colonic population of Escherichia coli, Clostridia, and Bifidobacteria. Lactobacilli count was significantly higher in the 0.2% MOS-fed group (9.77 ± 0.12) compared with the CON group (9.19 ± 0.16). Mannan-oligosaccharide did not affect the immune response of the birds as antibody titer against the New Castle disease virus and the cell-mediated immunity remained similar in all the treatment groups. In conclusion, dietary MOS supplementation influenced only the colonic lactobacilli population without any apparent effects on the production performance in growing pigeons.
益生元作为抗生素生长促进剂的替代品在禽类行业中得到了不同程度的应用。本试验旨在研究日粮甘露寡糖（MOS）添加对母源隔离岩鸽生长性能、结肠菌群和免疫反应的影响。将 36 只鸽子（n = 36）随机分为 4 个处理组（每组 9 只，每组 3 个重复或围栏），用玉米/小麦基础日粮（对照组/CON）或相同日粮添加 0.1%、0.2%或 0.5% MOS 喂养 35 d。在第 35 天，处死鸽子以收集内脏器官、结肠内容物和血清。结肠内容物用于计数选定的微生物群，血清用于检测抗新城疫病毒的抗体滴度。通过测量 2,4-二硝基氯苯（DNCB）挑战后的皮肤厚度来确定细胞介导的免疫。结果表明，添加物对鸽子的 BW 没有影响。在第 4 周，0.2%（187.9 ± 0.86）和 0.5%（186.4 ± 0.86）MOS 补充组的采食量明显高于 CON 组（180.7 ± 0.86）。与 CON 组（7.56 ± 0.33 和 6.25 ± 0.26）相比，MOS 0.1%（10.67 ± 0.33 和 8.22 ± 0.26）和 MOS 0.2%（9.91 ± 0.33 和 7.94 ± 0.26）组的肌胃重量（带和不带食糜）明显更高。与 CON 组（9.56 ± 0.56）相比，MOS 0.2%组（14.71 ± 0.56）的小肠重量明显更高。与 CON 组相比，所有 MOS 喂养组的小肠长度（MOS 0.1%=92.56 ± 1.69，MOS 0.2%=90.79 ± 1.69，MOS 0.5%=90.57 ± 1.69）和大肠长度在 MOS 0.1%（3.50 ± 0.02）和 MOS 0.5%（3.47 ± 0.02）喂养组中显著更高。心脏、肝脏、胰腺和大肠的重量不受影响。MOS 喂养不影响大肠杆菌、梭菌和双歧杆菌的结肠菌群。与 CON 组（9.19 ± 0.16）相比，MOS 0.2%喂养组的乳酸菌计数（9.77 ± 0.12）显著更高。甘露寡糖对禽类的免疫反应没有影响，新城疫病毒抗体滴度和细胞介导的免疫反应在所有治疗组中保持相似。综上所述，日粮 MOS 添加仅影响结肠乳酸菌群，而对生长鸽子的生产性能没有明显影响。
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